Optical analysis of liquid mixing in a minibioreactor.
Optical analysis of liquid mixing in a minibioreactor.
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微型生物反应器中液体混合的光学分析。
DOI：
10.1002/bit.20785
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Rao,Govind
中科院分区：
文献类型：
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作者：
Vallejos,JoseR;Kostov,Yordan;Ram,Arun;French,JosephA;Marten,MarkR;Rao,Govind
一种新型的光学传感器被用来研究混合和平均循环时间在一个模型微型生物反应器（12.5毫升搅拌容器，配备了桨式叶轮）。研究了10- 1，000 rpm范围内的旋转速率，对应于14和1，350之间的雷诺数。结果表明，根据叶轮转速，可重复实现高达214 ± 87 s的混合时间。微型生物反应器在过渡状态下操作，并且确定混合时间的无量纲形式NΘ M线性依赖于雷诺数。还确定了平均循环时间与转速倒数之间的线性相关性。12.5 mL搅拌容器中平均循环时间对转速的依赖性与大规模搅拌容器中的结果相似。这些结果表明，在大型反应器中发现的混合和循环时间可以在微型生物反应器中复制。© 2005 Wiley Periodicals，Inc.
A novel optical sensor was used to study mixing and mean circulation time in a model minibioreactor (12.5 mL stirred vessel, equipped with a paddle impeller). Rotational rates in the range of 10–1,000 rpm corresponding to Reynolds number between 14 and 1,350 were studied. Results suggest that depending on the impeller rotational speed, mixing times up to 214 ± 87 s can be reproducibly achieved. The minibioreactor was operated in the transitional regime, and it was determined that the non‐dimensional form for mixing time, NΘMwas linearly dependent on Reynolds number. A linear correlation between mean circulation time and the inverse of rotational speed was also determined. The mean circulation time dependence on rotational speed in the 12.5 mL stirred vessel is similar to those found in large‐scale stirred vessels. These results suggest that mixing and circulation times found in large‐scale reactors can be replicated in minibioreactors. © 2005 Wiley Periodicals, Inc.