Role of Microvesicles From Bone Marrow Mesenchymal Stem Cells in Acute Pancreatitis
Role of Microvesicles From Bone Marrow Mesenchymal Stem Cells in Acute Pancreatitis
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骨髓间充质干细胞微泡在急性胰腺炎中的作用
DOI：
10.1097/mpa.0000000000000694
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发表时间：
2016-10
期刊：
影响因子：
2.9
通讯作者：
Wang Xingpeng
中科院分区：
文献类型：
--
作者：
Yin Guojian;Hu Guoyong;Wan Rong;Yu Ge;Cang Xiaofeng;Xiong Jie;Ni Jianbo;Hu Yanling;Xing Miao;Fan Yuting;Xiao Wenqin;Qiu Lei;Tang Maochun;Zhao Yan;Wang Shaofeng;Wang Xingpeng
目的骨髓间充质干细胞（MSCs）对急性胰腺炎（AP）有明显的保护作用。本研究旨在分析骨髓间充质干细胞衍生微泡（bmMSC-MV）对AP的影响并探讨其可能机制。方法分离培养SD大鼠骨髓间充质干细胞（MSCs）和骨髓间充质干细胞微血管（bmMSC-MV）。采用蛙皮素诱导的轻度AP（MAP）和牛磺胆酸钠诱导的重度AP（SAP）作为体内和体外AP模型。通过测量血清淀粉酶、脂肪酶、趋化因子和白细胞介素水平，以及胰腺组织学、逆转录-聚合酶链反应、蛋白质印迹和免疫组织化学来评估胰腺损伤。体外培养胰腺腺泡细胞，观察bmMSC-MVs对胰腺腺泡细胞存活率的影响。结果骨髓MSC-MVs通过调节IL-1、IL-6、TNF-α的表达减轻MAP和SAP大鼠急性胰腺损伤，并显著抑制NF- B p65核转位。在MAP和SAP模型中，骨髓MSC-MV可提高胰腺腺泡细胞的存活率。结论骨髓MSC-MVs通过降低AP患者血清中促炎细胞因子水平，调节NF- B p65核转位，对AP患者具有保护作用。骨髓间充质干细胞微血管可作为临床治疗SAP的一种策略。
Objectives Mesenchymal stem cells (MSCs) have shown an obvious protective effect on acute pancreatitis (AP). The purpose of the present study was to analyze the effect of bone marrow MSC-derived microvesicles (bmMSC-MVs) on AP and explore the underlying mechanisms. Methods Bone marrow MSCs and bmMSC-MVs were isolated from Sprague-Dawley rats. Cerulein-induced mild AP (MAP) and sodium taurocholate-induced severe AP (SAP) were used as AP models in vivo and in vitro. Pancreatic injury was evaluated by measuring serum levels of amylase, lipase, chemokines, and interleukins, and by pancreatic histology, reverse transcription-polymerase chain reaction, Western blotting, and immunohistochemistry. The effects of bmMSC-MVs on the survival rates of pancreatic acinar cells in vitro were also assessed. Results Bone marrow MSC-MVs attenuated acute pancreatic injury in MAP and SAP by regulating IL-1&agr;, IL-6, and TNF-&agr;, and dramatically attenuated the nuclear translocation of NF-&kgr;B p65 in MAP and SAP. Bone marrow MSC-MVs improved the survival rates of pancreatic acinar cells in MAP and SAP models in vitro. Conclusions Bone marrow MSC-MVs played a protective role in AP by reducing the levels of proinflammatory cytokines and regulating the nuclear translocation of NF-&kgr;B p65. Bone marrow MSC-MVs could be developed as a strategy for the clinical treatment of SAP.
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DOI：
10.1681/asn.2011050503
发表时间：
2012-02
期刊：
Journal of the American Society of Nephrology : JASN
影响因子：
--
作者：
Sang-Won Park;Mihwa Kim;K. Brown;D. VivetteD.;Agati;H. Lee
通讯作者：
Sang-Won Park;Mihwa Kim;K. Brown;D. VivetteD.;Agati;H. Lee
影响因子：
5.2
作者：
Zhu, Ying-gang;Feng, Xiao-mei;Abbott, Jason;Fang, Xiao-hui;Hao, Qi;Monsel, Antoine;Qu, Jie-ming;Matthay, Michael A.;Lee, Jae W.
通讯作者：
Lee, Jae W.
影响因子：
3.7
作者：
Bruno S;Grange C;Collino F;Deregibus MC;Cantaluppi V;Biancone L;Tetta C;Camussi G
通讯作者：
Camussi G
影响因子：
1.4
作者：
Guojian Yin;Guoyong Hu;Rong Wan;Ge Yu;Xiaofeng Cang;Jianbo Ni;J. Xiong;Y. Hu;M. Xing;Y. Fan;W. Xiao;Lei Qiu;Shaofeng Wang;Xingpeng Wang
通讯作者：
Guojian Yin;Guoyong Hu;Rong Wan;Ge Yu;Xiaofeng Cang;Jianbo Ni;J. Xiong;Y. Hu;M. Xing;Y. Fan;W. Xiao;Lei Qiu;Shaofeng Wang;Xingpeng Wang
影响因子：
6.1
作者：
Gatti, Stefano;Bruno, Stefania;Camussi, Giovanni
通讯作者：
Camussi, Giovanni