Analysis of K-ras, N-ras, H-ras, and p53 in lung neuroendocrine neoplasms.
Analysis of K-ras, N-ras, H-ras, and p53 in lung neuroendocrine neoplasms.
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DOI：
10.1097/00019606-199906000-00003
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发表时间：
1999-06
期刊：
影响因子：
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通讯作者：
M. Couce;D. Bautista;J. Costa;D. Carter
中科院分区：
文献类型：
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作者：
M. Couce;D. Bautista;J. Costa;D. Carter
本研究对11例典型类癌(TC)、4例非典型类癌(AC)、1例大细胞神经内分泌癌(LCNEC)和4例转移癌组织进行了p53基因外显子5~8、K-ras外显子1和2的点突变筛查。用聚合酶链式反应-单链构象多态(SSCP)和直接测序及免疫组织化学方法检测H-ras和N-ras基因的表达。K-ras基因外显子1在两例低度AC和其中一例转移瘤(分别为GAT12和AGT12)中发生突变。未发现N-ras和H-ras突变。在高级别AC和LCNEC中发现了p53基因外显子5和8的突变。3例P53免疫组织化学染色阳性，仅1例LCNEC基因突变。总之，P53基因点突变在这些肺神经内分泌肿瘤中并不常见，并不是所有的样本都与免疫组织化学染色相关，而是与较高级别的肿瘤相关。第二，K-ras基因突变的存在似乎与高级别的癌有关。在这些肺神经内分泌肿瘤中未发现N-ras和H-ras突变。
This study screened 11 samples of typical carcinoid (TC), 4 samples of atypical carcinoid (AC), 1 sample of large cell neuroendocrine carcinoma (LCNEC), and four metastases for point mutations in exons 5 to 8 of the p53 gene, and exons 1 and 2 of the K-ras. H-ras, and N-ras genes using polymerase chain reaction (PCR)-single-strand conformation polymorphism (SSCP) and direct sequencing and by immunohistochemistry for p53. Exon 1 of K-ras was mutated in two samples of low-grade AC and a metastasis from one of these tumors (GAT12 and AGT12, respectively). No mutations in N-ras or H-ras were found. Mutations in exons 5 and 8 of the p53 gene were identified in a high-grade AC and a LCNEC. Positive immunostaining for p53 was present in three samples, with only one genotypic mutation shown (LCNEC). In conclusion, point mutations of the p53 gene were infrequent in these pulmonary neuroendocrine tumors, did not correlate in all samples with immunostaining, and were associated with the higher-grade tumors. Second, the presence of K-ras mutations seems to be associated with the higher-grade carcinomas. Third, N-ras and H-ras mutations were not found with these pulmonary neuroendocrine tumors.