Photoactivated Carbon Dots for Inactivation of Foodborne Pathogens Listeria and Salmonella
Photoactivated Carbon Dots for Inactivation of Foodborne Pathogens Listeria and Salmonella
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DOI：
10.1128/aem.01042-21
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发表时间：
2021-12-01
影响因子：
4.4
通讯作者：
Yang, Liju
中科院分区：
文献类型：
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作者：
Dong, Xiuli;Wang, Ping;Yang, Liju
长期以来，食源性病原体一直被认为是食品工业面临的主要挑战，并一再与食品召回和食源性疾病爆发有关。这项研究展示了最近发现的一类可见光活性炭基纳米颗粒，即碳点（CDots）在食源性病原体光动力失活中的应用。结果表明，CDots对单增李斯特菌在悬浮液和不锈钢表面的光灭活效果良好。然而，它对沙门氏菌细胞的效果要差得多，但较高的CDot浓度和较长的时间处理仍然能够灭活沙门氏菌细胞。研究人员评估了CDots对两种类型细胞的不同抑菌作用的机制，并分析了处理后细胞内活性氧（ROS）的产生、脂质过氧化以及核酸和蛋白质的泄漏，结果表明CDots是一类有前途的食源性病原体光动力灭活剂。食源性传染病长期以来一直被认为是公共卫生的主要挑战。食品加工设施和设备经常受到食源性病原体的污染。迫切需要新的工具/方法来控制病原体并防止食品加工设施和其他环境中的此类污染。本研究报告了一种新建立的抗菌纳米材料平台（Dots与可见光/自然光耦合），用于有效和高效地灭活代表性食源性细菌病原体。该研究将有助于促进CDots作为一种新型的、有发展前景的基于纳米材料的食源性致病菌光动力灭活剂的实际应用。
Foodborne pathogens have long been recognized as major challenges for the food industry and repeatedly implicated in food product recalls and outbreaks of foodborne diseases. This study demonstrated the application of a recently discovered class of visible-light-activated carbon-based nanoparticles, namely, carbon dots (CDots), for photodynamic inactivation of foodborne pathogens. The results demonstrated that CDots were highly effective in the photoinactivation of Listeria monocytogenes in suspensions and on stainless steel surfaces. However, it was much less effective for Salmonella cells, but treatments with higher CDot concentrations and longer times were still able to inactivate Salmonella cells. The mechanistic implications of the observed different antibacterial effects on the two types of cells were assessed, and the associated generation of intracellular reactive oxygen species (ROS), the resulting lipid peroxidation, and the leakage of nucleic acid and proteins from the treated cells were analyzed, with the results collectively suggesting CDots as a class of promising photodynamic inactivation agents for foodborne pathogens.IMPORTANCE Foodborne infectious diseases have long been recognized as major challenges in public health. Contaminations of food processing facilities and equipment with foodborne pathogens occur often. There is a critical need for new tools/approaches to control the pathogens and prevent such contaminations in food processing facilities and other settings. This study reports a newly established antimicrobial nanomaterials platform, (Dots coupled with visible/natural light, for effective and efficient inactivation of representative foodborne bacterial pathogens. The study will contribute to promoting the practical application of CDots as a new class of promising nanomaterial-based photodynamic inactivation agents for foodborne pathogens.