A structured retroviral RNA element that mediates nucleocytoplasmic export of intron-containing RNA
A structured retroviral RNA element that mediates nucleocytoplasmic export of intron-containing RNA
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DOI：
10.1128/mcb.17.1.135
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发表时间：
1997-01-01
影响因子：
5.3
通讯作者：
Hammarskjold, ML
中科院分区：
文献类型：
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作者：
Ernst, RK;Bray, M;Hammarskjold, ML
所有逆转录病毒基因表达的一个共同特征是，尽管含有内含子的细胞RNA通常局限于细胞核，但未剪接的含内含子的RNA被输出到细胞质中。在复杂的逆转录病毒中，含有内含子的RNA的输出是由特定的病毒调节蛋白介导的（例如，人类免疫缺陷病毒1型[HIV-1] Rev），它与病毒RNA中的元素结合。然而，较简单的逆转录病毒不编码这种调节蛋白。在这里，我们展示了更简单的逆转录病毒梅森-辉瑞猴病毒（MPMV）的基因组包含一个元素，作为含内含子RNA的自主核输出信号。该元素对MPMV复制至关重要；然而，它的功能可以由HIV-I Rev和Rev-responsive元件来补充。该元素还可以促进细胞含内含子RNA的输出。这些结果表明MPMV元件通过与内源性细胞因子的相互作用模拟细胞RNA转运信号并介导RNA输出。
A common feature of gene expression in all retroviruses is that unspliced, intron-containing RNA is exported to the cytoplasm despite the fact that cellular RNAs which contain introns are usually restricted to the nucleus. In complex retroviruses, the export of intron-containing RNA is mediated by specific viral regulatory proteins (e.g., human immunodeficiency virus type 1 [HIV-1] Rev) that bind to elements in the viral RNA. However, simpler retroviruses do not encode such regulatory proteins. Here,ve show that the genome of the simpler retrovirus Mason-Pfizer monkey virus (MPMV) contains an element that serves as an autonomous nuclear export signal for intron-containing RNA. This element is essential for MPMV replication; however, its function can be complemented by HIV-I Rev and the Rev-responsive element. The element can also facilitate the export of cellular intron-containing RNA. These results suggest that the MPMV element mimics cellular RNA transport signals and mediates RNA export through interaction with endogenous cellular factors.