ENDOTHELIN-1 STIMULATION OF NORADRENALINE AND ADRENALINE RELEASE FROM ADRENAL CHROMAFFIN CELLS
ENDOTHELIN-1 STIMULATION OF NORADRENALINE AND ADRENALINE RELEASE FROM ADRENAL CHROMAFFIN CELLS
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DOI：
10.1016/0006-2952(91)90623-d
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发表时间：
1991-02-15
影响因子：
5.8
通讯作者：
MARRIOTT, DB
中科院分区：
文献类型：
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作者：
BOARDER, MR;MARRIOTT, DB
内皮素-1(ET-1)刺激牛肾上腺嗜铬细胞释放去甲肾上腺素和肾上腺素，与50 mM钾相比，刺激释放较小。Sarafooxin-6b刺激释放的程度与ET-1相似。ET-1刺激释放的EC50约为1 nM。这种钙依赖性释放可被尼群地平(1-mU-M)部分抑制，但与钙通道激动剂Bay K 8644(1-mU-M)无协同作用。次极量钾刺激与ET-1联合应用时也未见协同释放作用。ET-1对Fura-2负载细胞的刺激产生了一种不寻常的反应时程，该时程缓慢上升至最大值，并保持不变。这些结果表明，ET-1对去甲肾上腺素和含肾上腺素的嗜铬细胞均有刺激作用，其作用机制部分依赖于二氢吡啶敏感的钙通道，但有别于钙通道激动剂或膜去极化。
Endothelin-1 (ET-1) stimulated release of both noradrenaline and adrenaline from cultured bovine adrenal chromaffin cells; stimulated release was small compared to that elicited by 50 mM potassium. Sarafotoxin-6b stimulated release to a similar extent as ET-1. The ET-1 stimulated release had an EC50 of about 1 nM. This calcium-dependent release was partially inhibited by nitrendipine (1-mu-M), but there was no synergistic interaction with the calcium channel agonist BAY K 8644 (1-mu-M). There was also no synergistic release seen when submaximal stimulation with potassium was combined with ET-1. Stimulation of fura-2 loaded cells with ET-1 produced an unusual timecourse of response which rose slowly to a maximum which was sustained. These results show that ET-1 may stimulate both noradrenaline and adrenaline containing chromaffin cells by a mechanism which, while partially dependent on dihydropyridine sensitive calcium channels, is distinct from the calcium channel agonist or membrane depolarization.