Reverse methanogenesis: Testing the hypothesis with environmental genomics
Reverse methanogenesis: Testing the hypothesis with environmental genomics
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DOI：
10.1126/science.1100025
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发表时间：
2004-09-03
期刊：
影响因子：
56.9
通讯作者：
DeLong, EF
中科院分区：
文献类型：
--
作者：
Hallam, SJ;Putnam, N;DeLong, EF
缺氧沉积物中微生物对甲烷的消耗通过减少海洋向大气的温室气体通量而对全球环境产生重要影响。尽管它的意义，控制厌氧甲烷氧化的生物机制没有得到很好的表征。目前的一个模型表明，产生甲烷的古细菌的亲属发展了逆转甲烷生成的能力，从而消耗甲烷来产生细胞碳和能量。我们在这里报告的“反向甲烷生成”假说的基因组分析的甲烷氧化菌，从深海沉积物的测试。我们的研究结果表明，几乎所有的基因通常与甲烷生产存在于一个特定的古菌甲烷氧化菌。这些基于基因组的观察支持了先前的假设，并为厌氧甲烷氧化的代谢建模提供了一个知情的基础。
Microbial methane consumption in anoxic sediments significantly impacts the global environment by reducing the flux of greenhouse gases from ocean to atmosphere. Despite its significance, the biological mechanisms controlling anaerobic methane oxidation are not well characterized. One current model suggests that relatives of methane-producing Archaea, developed the capacity to reverse methanogenesis and thereby to consume methane to produce cellular carbon and energy. We report here a test of the "reverse-methanogenesis" hypothesis by genomic analyses of methane-oxidizing Archaea, from deep-sea sediments. Our results show that nearly all genes typically associated with methane production are present in one specific group of archaeal methanotrophs. These genome-based observations support previous hypotheses and provide an informed foundation for metabolic modeling of anaerobic methane oxidation.