Synthesis and application of mono-6-ammonium-6-deoxy-beta-cyclodextrin chloride as chiral selector for capillary electrophoresis.
Synthesis and application of mono-6-ammonium-6-deoxy-beta-cyclodextrin chloride as chiral selector for capillary electrophoresis.
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DOI：
10.1016/j.chroma.2005.07.082
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发表时间：
2005-11
期刊：
影响因子：
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通讯作者：
Weihua Tang;I. Muderawan;Siu-Choon Ng;Hardy Sze On Chan
中科院分区：
文献类型：
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作者：
Weihua Tang;I. Muderawan;Siu-Choon Ng;Hardy Sze On Chan
提出了一种简便的单-6-氨基-6-脱氧-β-环糊精(β-CD-NH_2)的合成方法。进一步合成了其羟基盐单-6-氨基-6-脱氧-β-环糊精氯化物(β-CD-NH_3Cl)，并用于毛细管电泳法分离各种阴离子和两性分析物。研究了背景电解液(BGE)、pH和选择剂浓度对拆分的影响。结果表明，β-CD-NH_3Cl对阴离子分析物具有较强的手性拆分能力。此外，在35min内实现了由8种酸组成的标准混合物的基线分离。
A facile synthetic approach for mono-6-amino-6-deoxy-β-cyclodextrin (β-CD-NH2) was proposed. Its hydroxy chloride salt, mono-6-ammonium-6-deoxy-β-cyclodextrin chloride (β-CD-NH3Cl) was further prepared and used for the enantioseparation of various anionic and ampholytic analytes by capillary electrophoresis (CE). The effect of background electrolyte (BGE) pH and selector concentration on the enantioseparation was studied. Results showed that β-CD-NH3Cl displayed powerful chiral resolution ability towards anionic analytes. In addition, baseline separation of a standard mixture consisting of eight acids was achieved within 35min.