Final grain weight is not limited by the activity of key starch-synthesising enzymes during grain filling in wheat.
Final grain weight is not limited by the activity of key starch-synthesising enzymes during grain filling in wheat.
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DOI：
10.1093/jxb/ery314
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发表时间：
2018-11-26
影响因子：
6.9
通讯作者：
Smith AM
中科院分区：
文献类型：
--
作者：
Fahy B;Siddiqui H;David LC;Powers SJ;Borrill P;Uauy C;Smith AM
在有利于高产的田间条件下，小麦的最终粒重不受灌浆期糖的可用性或淀粉合成关键酶活性的限制。由于淀粉是成熟小麦籽粒的主要组成部分，因此人们认为灌浆过程中淀粉合成能力的变化会影响最终的粒重。我们研究了这一假设，通过研究总共54个小麦基因型，包括精英品种和地方品种，在英格兰东部的田地里连续两年种植。在灌浆过程中，测定了籽粒重量、含水量、糖、淀粉和淀粉合成的两种酶，ADP-葡萄糖焦磷酸化酶和可溶性淀粉合成酶的最大催化活性。分析了这些变量与成熟籽粒重量和淀粉含量的关系。最终粒重表现出很少或没有显着的相关性与酶活性，糖水平，或在灌浆过程中的淀粉含量，或在成熟时的淀粉含量。我们的结论是，在我们的田间条件下，无论是糖的可用性，也没有淀粉合成的酶的能力在籽粒灌浆显着影响最终粒重。我们认为，最终粒重可能在很大程度上决定于灌浆前的发育过程。淀粉的积累使籽粒达到发育过程所设定的物理极限。这一结论与以往人为操纵源汇强度的研究结果雅阁。
In field conditions that favour high yields, final grain weight of wheat is not limited by sugar availability or activities of key enzymes of starch synthesis during grain filling. Since starch is by far the major component of the mature wheat grain, it has been assumed that variation in the capacity for starch synthesis during grain filling can influence final grain weight. We investigated this assumption by studying a total of 54 wheat genotypes including elite varieties and landraces that were grown in two successive years in fields in the east of England. The weight, water content, sugars, starch, and maximum catalytic activities of two enzymes of starch biosynthesis, ADP-glucose pyrophosphorylase and soluble starch synthase, were measured during grain filling. The relationships between these variables and the weights and starch contents of mature grains were analysed. Final grain weight showed few or no significant correlations with enzyme activities, sugar levels, or starch content during grain filling, or with starch content at maturity. We conclude that neither sugar availability nor enzymatic capacity for starch synthesis during grain filling significantly influenced final grain weight in our field conditions. We suggest that final grain weight may be largely determined by developmental processes prior to grain filling. Starch accumulation then fills the grain to a physical limit set by developmental processes. This conclusion is in accord with those from previous studies in which source or sink strength has been artificially manipulated.
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影响因子：
7.2
作者：
Delatte, T;Trevisan, M;Zeeman, SC
通讯作者：
Zeeman, SC
影响因子：
5.8
作者：
Ferrante, Ariel;Savin, Roxana;Slafer, Gustavo A.
通讯作者：
Slafer, Gustavo A.
影响因子：
4.2
作者：
JENNER, CF;RATHJEN, AJ
通讯作者：
RATHJEN, AJ
DOI：
10.1071/pp9930197
发表时间：
1993-01-01
期刊：
AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY
影响因子：
--
作者：
HAWKER, JS;JENNER, CF
通讯作者：
JENNER, CF
DOI：
10.1071/pp9940783
发表时间：
1994-01-01
期刊：
AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY
影响因子：
--
作者：
DENYER, K;HYLTON, CM;SMITH, AM
通讯作者：
SMITH, AM