OXIDATION - IMPORTANT ENZYME REACTION IN FUNGAL DEGRADATION OF CELLULOSE
OXIDATION - IMPORTANT ENZYME REACTION IN FUNGAL DEGRADATION OF CELLULOSE
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DOI：
10.1016/0014-5793(74)80531-4
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发表时间：
1974-01-01
期刊：
影响因子：
3.5
通讯作者：
WESTERMARK, U
中科院分区：
文献类型：
--
作者：
ERIKSSON, KE;PETTERSSON, B;WESTERMARK, U
粉状孢子菌用于降解纤维素的胞外酶系统已在一系列论文中报道[l--5]。从以粉末纤维素为唯一碳源培养该真菌获得的培养液中，纯化并表征了五种内切-l/l-&葡聚糖酶和一种外切-1, 4-p-葡聚糖酶，并证明了这些内切和外切葡聚糖酶之间的强协同作用。最近从S. pulverulentum的培养液中分离出另一种酶，纤维二糖：醌氧化还原酶[6-81。这种酶在纤维二糖存在的情况下还原醌，纤维二糖又被氧化成纤维二酸内酯。该酶被认为在纤维素和木质素降解中都很重要 [9, 10]。在这种情况下，醌是由酚氧化酶产生的，酚氧化酶氧化木质素降解过程中产生的酚。我们现在在 S. pulverulentum 培养液中发现了另一种酶，它对纤维素降解很重要。该酶氧化纤维素，并且在纤维素降解过程中它的存在产生的降解程度至少是当氧化酶不存在时内切和外切葡聚糖酶的相同混合物所产生的降解程度的两倍。
The extracellular enzyme stystem utilized by Sporotrichum pulverulentum for the degradation of cellulose has been reported in a series of papers [l--5]. From the culture solution obtained by growing this fungus on powder cellulose as the sole carbon source, five endo-l/l-&lucanases and one exo-1, 4-/?-glucanase have been purirled and characterized and a strong synergistic action between these endo-and exoglucanases demonstrated. Recently another enzyme, cellobiose: quinone oxidoreductase, was isolated from the culture solution of S. pulverulentum [6-81. This enzyme reduces quinones in the presence of cellobiose which is in turn oxidized to cellobionos-lactone. The enzyme is thought to be of importance in both cellulose and lignin degradation [9, 10]. In this case the quinones are produced by phenol oxidases which oxidize phenols produced during lignin degradation. We have now discovered yet another enzyme in the culture solution of S. pulverulentum which is important for cellulose degradation. The enzyme oxidizes cellulose and its presence during cellulose degradation gives at least twice the extent of degradation given by the same mixture of endo-and exo-glucanases when the oxidizing enzyme is absent.