Phase variation of Coxiella burnetii strain Priscilla:: influence of this phenomenon on biochemical features of its lipopolysaccharide
Phase variation of Coxiella burnetii strain Priscilla:: influence of this phenomenon on biochemical features of its lipopolysaccharide
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DOI：
10.1177/09680519000060050701
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发表时间：
2000-01-01
期刊：
影响因子：
--
通讯作者：
Toman, R
中科院分区：
文献类型：
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作者：
Ftácek, P;Skultéty, L;Toman, R
在伯氏柯克斯体的相位变化过程中，研究了其脂多糖（LPS）成分的变化。克隆的 I 期伯内特氏细胞在鸡胚卵黄囊中连续传代，直至卵传代 (EP) 90。来自 EP 3、12、21、40、60 和 90 中细胞的 LPS 均通过空间排阻色谱法分为三个主要群体：高、中和低分子量部分，其大小和化学组成彼此不同。在 C. burnetii 培养过程中分离的 LPS 中没有观察到 O-多糖链明显缩短。然而，由于整个细胞群中表达截断 O 链和 R 型 LPS 分子的细胞越来越普遍，已经观察到现有 LPS 群体的重新分布。在高分子量和中分子量LPS群体中，病毒糖和二氢羟基链霉糖随着相变的进展而逐渐损失。对于前一种糖，这种情况更容易发生。目前，影响伯内氏相变过程中LPS修饰的分子机制仍不清楚。
During the phase variation of Coxiella burnetii, modifications in its lipopolysaccharide (LPS) component were investigated. The cloned phase I C. burnetii cells were passed serially in chicken embryo yolk sacs up to the egg passage (EP) 90. The LPSs from the cells in EPs 3, 12, 21, 40, 60, and 90 were all separated by steric exclusion chromatography into three major populations: the high, intermediate, and low molecular weight fractions, differing one from another in size and chemical composition. No noticeable shortening of the O-polysaccharide chains was observed in the LPSs isolated during the C. burnetii cultivation. However, a redistribution of the existing LPS populations has been observed due to an increasing prevalence of those cells in the whole cell population that express LPS molecules with truncated O-chains and those being of R-type. In the high and intermediate molecular weight LPS populations, virenose and dihydrohydroxystreptose are lost gradually with the progress in phase variation. This occurs more readily with the former sugar. At present, the molecular mechanisms influencing the LPS modifications during the C. burnetii phase variation remain unclear.