[Effect of vinyl chloride on reproductive and endocrine system of male rats].
[Effect of vinyl chloride on reproductive and endocrine system of male rats].
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DOI：
10.3760/cma.j.issn.1001-9391.2010.07.010
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发表时间：
2010-07
影响因子：
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通讯作者：
Xiao-xiao Wang;Jing-wei Xiao;Hui-lin Meng;T. Cui;Kailong Niu;Bin Li
中科院分区：
文献类型：
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作者：
Xiao-xiao Wang;Jing-wei Xiao;Hui-lin Meng;T. Cui;Kailong Niu;Bin Li
目的探讨氯乙烯对雄性大鼠生殖内分泌系统的影响。方法雄性SD大鼠分别以0、10、100、1000 mg/kg的剂量染毒14和28 d。测定血清和睾丸组织匀浆中睾酮（T）、卵泡刺激素（FSH）、雌二醇（E2）、促黄体生成素（LH）、卵泡刺激素（FSH）、卵泡刺激素（B）B的水平。取睾丸组织行电镜检查。结果与对照组相比，染毒14 d后，1000、100、10 mg/kg组血清T、E2水平降低，3个剂量组InhB、LH水平升高。100 mg/kg组血清LH水平显著高于对照组（P < 0.05）。染毒28 d后，100、1000 mg/kg组血清T水平分别为（10.90 ± 1.56）、（8.52 ± 2.85）ng/ml（P < 0.05），100、1000 mg/kg组血清InhB水平分别为（31.40 ± 6.21）、（28.39 ± 5.67）pg/ml。100、1000 mg/kg组血清T和InhB水平均显著降低（P < 0.05）。10、100、1000 mg/kg组血清FSH水平较对照组显著降低（P < 0.05）。10、100、1000 mg/kg剂量组28 d后血清InhB和LH水平较14 d组显著降低（P <0.05）。100、1000 mg/kg组睾丸T和InhB水平分别为8.05 ± 2.19、6.75 ± 1.94 ng/mg pro和39.32 ± 5.55、35.53 ± 8.71 pg/mg pro，与对照组相比显著降低（P < 0.05）。组织病理学检查示睾丸间质细胞和支持细胞受损。结论氯乙烯对雄性大鼠生殖内分泌系统有不良影响，可引起大鼠血清和睾丸匀浆激素水平的改变。
OBJECTIVE To explore the effect of vinyl chloride on reproductive and endocrine system of male rats. METHODS Male SD rats were administered with vinyl chloride at dose of (0, 10, 100, 1000 mg/kg) for 14 and 28 days, respectively. The levels of testosterone (T), inhibin B, luteinizing hormone (LH), follicle stimulating hormone (FSH) and estradiol (E2) were measured in serum and testis homogenates. Histopathological examinations were performed for testis with electron microscopy. RESULTS Compared with the control group after 14-day exposure, T and E2 serum levels of 1000, 100, 10 mg/kg groups decreased, InhB and LH levels of three dose groups increased. LH serum levels of 100 mg/kg increased significantly statistically compared with control group (P < 0.05). After 28-day exposure, T serum levels of 100, 1000 mg/kg groups were (10.90 +/- 1.56), (8.52 +/- 2.85) ng/ml respectively (P < 0.05), InhB serum levels of 100, 1000 mg/kg groups were (31.40 +/- 6.21), (28.39 +/- 5.67) pg/ml respectively. Both of T and InhB serum levels of 100, 1000 mg/kg groups decreased significantly (P < 0.05). Serum FSH levels of 10, 100, 1000 mg/kg groups decreased significantly compared with control group (P < 0.05). Compared with groups of 14-day exposure, serum InhB and LH levels of 10, 100, 1000 mg/kg groups decreased significantly statistically after 28 days. T and InhB testis levels of 100, 1000 mg/kg groups were 8.05 +/- 2.19),(6.75 +/- 1.94) ng/mg pro and (39.32 +/- 5.55), (35.53 +/- 8.71) pg/mg pro respectively, which decreased significantly compared with control group (P < 0.05). Leydig cell and Sertoli cell were damaged according to histopathological examinations. CONCLUSION Vinyl chloride has adverse effects on reproductive and endocrine system of male rats and may change their serum and testis homogenate levels of hormones.