Matrix Gla Protein Expression in NRK-52E Cells Exposed to Oxalate and Calcium Oxalate Monohydrate Crystals
Matrix Gla Protein Expression in NRK-52E Cells Exposed to Oxalate and Calcium Oxalate Monohydrate Crystals
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DOI：
10.1159/000314947
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发表时间：
2010-01-01
影响因子：
1.6
通讯作者：
Kohri, Kenjiro
中科院分区：
文献类型：
--
作者：
Gao, Bing;Yasui, Takahiro;Kohri, Kenjiro
目的：基质Gla蛋白（MGP）是细胞外基质中一种有效的钙化抑制剂，是调节血管钙化的分子决定因子。在这里，我们的特点是MGP表达在培养的肾小管上皮细胞暴露于草酸盐和草酸钙一水合物（COM）晶体。方法：体外培养NRK-52 E细胞，分别用草酸盐和COM处理1、3、6、12和24 h。采用实时荧光定量PCR（RT-PCR）检测MGP mRNA的表达水平，Western blot检测MGP蛋白的表达水平。结果：MGP在COM作用后表达迅速而强烈，并与草酸作用时间相关。结论：我们的研究结果给出了暴露于草酸盐和晶体的肾小管上皮细胞中MGP表达的概况，并为MGP和肾结石之间的关联提供了支持。因此，进一步研究MGP在晶体-细胞相互作用过程中的作用机制具有重要意义。版权所有（C）2010 S. Karger AG，巴塞尔
Purpose: Matrix Gla protein (MGP), a potent calcification inhibitor in extracellular matrix, is a molecular determinant regulating vascular calcification. Here, we characterized MGP expression in cultured renal tubular epithelial cells exposed to oxalate and calcium oxalate monohydrate (COM) crystals. Methods: NRK-52E cells were cultured and exposed to medium containing oxalate or COM for 1, 3, 6, 12 and 24 h. The levels of MGP mRNA expression were quantified using real-time PCR (RT-PCR), and the levels of protein expression were characterized by Western blot. Results: MGP was expressed rapidly and intensively following exposure to COM and was time-dependent with exposure to oxalate. Conclusion: Our results have given a profile of MGP expression in renal tubular epithelial cells exposed to oxalate and crystals and provided support for an association between MGP and kidney stones. Further investigations on the mechanism of the MGP in the process of crystal-cell interaction may be important in the future. Copyright (C) 2010 S. Karger AG, Basel