Innervation of periodontal ligament and dental pulp in the rat incisor: An immunohistochemical investigation of neurofilament protein and glia-specific S-100 protein
Innervation of periodontal ligament and dental pulp in the rat incisor: An immunohistochemical investigation of neurofilament protein and glia-specific S-100 protein
复制标题
大鼠门牙牙周膜和牙髓的神经支配：神经丝蛋白和神经胶质特异性 S-100 蛋白的免疫组织化学研究
DOI：
10.1007/bf00215442
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发表时间：
2004
影响因子：
3.6
通讯作者：
Y. Takahashi
中科院分区：
文献类型：
--
作者：
O. Sato;T. Maeda;Shigeo Kobayashi;T. Iwanaga;T. Fujita;Y. Takahashi
本研究采用神经丝蛋白（NFP）和胶质细胞特异性S-100蛋白免疫组化方法，对大鼠切牙牙周膜和牙髓中的神经成分进行了研究。在切牙牙周膜密集的NFP免疫反应阳性神经纤维的神经纤维的分布和它们的终端显着不同的磨牙。NFP阳性的粗神经束通过位于牙槽窝中部的缝隙进入舌侧牙周膜，并立即形成大量的Ruffini样小体。在唇侧牙周膜中，所有NFP免疫反应阳性神经纤维终止于游离终末。在舌侧牙周膜中，牵张感受器（Ruffini样小体）的受限位置似乎是一个重要因素，因为该区域在咀嚼过程中经常延伸。神经元在每个区域都局限于牙周膜的牙槽骨部分;它们避开了牙周膜的牙侧部分，因为牙侧部分可能会随着牙齿不断移动。切牙牙髓神经纤维的分布也有不同于磨牙的特征;神经纤维呈串珠状分布于牙髓中央向切缘，不形成成牙本质细胞下神经丛，S-100蛋白免疫组化显示神经元的分布模式与NFP相似，提示供应牙周膜和牙髓的神经大部分被雪旺氏鞘覆盖。
SummaryNervous elements in the periodontal ligament and dental pulp of rat incisors were investigated by means of immunohistochemistry for neurofilament protein (NFP) and glia-specific S-100 protein. The periodontal ligament in the incisors was densely innervated by NFP-immunoreactive nerve fibers; the distribution of the nerve fibers and their terminations differed markedly from those in molars. NFP-positive, thick nerve bundles entered the lingual periodontal ligament through slits located in the mid-region of the alveolar socket, and immediately formed numerous Ruffini-like corpuscles. In the labial periodontal ligament, all of the NFP-immunoreactive nerve fibers terminated in free endings. The restricted location of the stretch receptor, Ruffini-like corpuscle, in the lingual periodontal ligament appears to be an essential element, because this region is regularly extended during mastication. The nervous elements were restricted to the alveolar half of the periodontal ligament in every region; they avoided the dental half of the periodontal ligament, which presumably moves continuously with the tooth. Pulpal nerve fibers in incisors also showed a characteristic distribution different from those in molars; individual nerve fibers with beaded structures ran in the center of the pulp toward the incisai edge, and did not form the subodontoblastic nerve plexus of Raschkow.Immunostaining for S-100 protein revealed a distribution pattern of nervous elements similar to that for NFP, suggesting that the nerves supplying the periodontal ligament and dental pulp were mostly covered by a Schwann sheath.