Tembusu-like flavivirus (Perak virus) as the cause of neurological disease outbreaks in young Pekin ducks
Tembusu-like flavivirus (Perak virus) as the cause of neurological disease outbreaks in young Pekin ducks
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DOI：
10.1080/03079457.2014.973832
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发表时间：
2014-11-02
期刊：
影响因子：
2.8
通讯作者：
Palya, Vilmos
中科院分区：
文献类型：
--
作者：
Homonnay, Zalan Gabor;Kovacs, Edit Walkone;Palya, Vilmos
2012年，在马来西亚的几个养鸭场观察到一种以共济失调、跛行和瘫痪为特征的年轻北京鸭神经系统疾病。在大多数病例中，肉眼病理学病变不存在或不一致，但在脑和脊髓中发现严重且一致的显微镜下病变，其特征为非化脓性全脑脊髓炎。在含胚鸭蛋和接种受感染鸭脑匀浆的细胞培养物（Vero和DF-1）中获得了几个病毒分离株。在排除其他病毒后，通过黄病毒特异性逆转录-聚合酶链反应（RT-PCR）测定将分离株鉴定为黄病毒。用黄病毒分离株经皮下或肌内途径接种2周龄北京鸭，在脑和脊髓中引起典型的临床症状和组织学病变。用RT-PCR方法从临床症状和组织学病变的鸭脏器中检测到病毒。延迟几天后，在混合接触控制禽类中也观察到了该疾病。NS 5和E基因序列的系统发育分析证明，分离物是一个新的系统发育组内的支XI（恩塔亚病毒组）的黄病毒属的代表。该马来西亚鸭坦布苏病毒（DTMUV），命名为霹雳州病毒，与在中国鉴定的一种相关DTMUV具有中度基因组RNA序列相似性。在我们的实验中，马来西亚的DTMUV株可以在没有蚊子媒介的情况下传播。这些发现可能对控制和预防这组新出现的黄病毒具有意义。
A neurological disease of young Pekin ducks characterized by ataxia, lameness, and paralysis was observed at several duck farms in Malaysia in 2012. Gross pathological lesions were absent or inconsistent in most of the cases, but severe and consistent microscopic lesions were found in the brain and spinal cord, characterized by non-purulent panencephalomyelitis. Several virus isolates were obtained in embryonated duck eggs and in cell cultures (Vero and DF-1) inoculated with the brain homogenates of affected ducks. After exclusion of other viruses, the isolates were identified as a flavivirus by flavivirus-specific reverse transcription-polymerase chain reaction (RT-PCR) assays. Inoculation of 2-week-old Pekin ducks with a flavivirus isolate by the subcutaneous or intramuscular route resulted in typical clinical signs and histological lesions in the brain and spinal cord. The inoculated virus was detected by RT-PCR from organ samples of ducks with clinical signs and histological lesions. With a few days delay, the disease was also observed among co-mingled contact control birds. Phylogenetic analysis of NS5 and E gene sequences proved that the isolates were representatives of a novel phylogenetic group within clade XI (Ntaya virus group) of the Flavivirus genus. This Malaysian Duck Tembusu Virus (DTMUV), named Perak virus, has moderate genomic RNA sequence similarity to a related DTMUV identified in China. In our experiment the Malaysian strain of DTMUV could be transmitted in the absence of mosquito vectors. These findings may have implications for the control and prevention of this emerging group of flaviviruses.