Complete Genome and Proteome of Acholeplasma laidlawii
Complete Genome and Proteome of Acholeplasma laidlawii
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DOI：
10.1128/jb.05059-11
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发表时间：
2011-09-01
影响因子：
3.2
通讯作者：
Govorun, V. M.
中科院分区：
文献类型：
--
作者：
Lazarev, V. N.;Levitskii, S. A.;Govorun, V. M.
本文报道了水蛭科水蛭科水蛭纲水蛭门PG-8A的全基因组序列和蛋白质基因组图谱。线虫基因组由一条1,496,992 bp的环状染色体代表，平均G + C含量为31 mol%。这是Mollicutes中已知核苷酸序列最长的基因组。它包含聚合酶I型、SOS反应和信号转导系统的基因，以及RNA调节元件、核糖开关和T盒。这证明了对基因表达的调控和对胁迫的诱变反应的显著能力。laidlawii和phytoplasma是已知的唯一使用通用遗传密码的Mollicutes，其中UGA是一个停止密码子。在Mollicutes组中，只有不需要甾醇的血浆具有从头合成饱和脂肪酸的能力。蛋白质组学数据用于基因组的初级注释，验证了许多预测蛋白质的表达。我们还检测到A. laidlawii蛋白的翻译后修饰：磷酸化和酰化。共发现74个候选磷酸化蛋白：16个候选蛋白是拟南芥特有的蛋白，11个候选蛋白是表面锚定蛋白或整体膜蛋白，这表明存在活跃的信号通路。在20个酰化蛋白中，14个含有棕榈酸链，6个含有硬脂酸链。未发现亚油酸和油酸的残留。酰基化蛋白主要是糖和无机离子运输系统的组成部分，是具有未知功能的表面锚定蛋白。
We present the complete genome sequence and proteogenomic map for Acholeplasma laidlawii PG-8A (class Mollicutes, order Acholeplasmatales, family Acholeplasmataceae). The genome of A. laidlawii is represented by a single 1,496,992-bp circular chromosome with an average G + C content of 31 mol%. This is the longest genome among the Mollicutes with a known nucleotide sequence. It contains genes of polymerase type I, SOS response, and signal transduction systems, as well as RNA regulatory elements, riboswitches, and T boxes. This demonstrates a significant capability for the regulation of gene expression and mutagenic response to stress. Acholeplasma laidlawii and phytoplasmas are the only Mollicutes known to use the universal genetic code, in which UGA is a stop codon. Within the Mollicutes group, only the sterol-nonrequiring Acholeplasma has the capacity to synthesize saturated fatty acids de novo. Proteomic data were used in the primary annotation of the genome, validating expression of many predicted proteins. We also detected posttranslational modifications of A. laidlawii proteins: phosphorylation and acylation. Seventy-four candidate phosphorylated proteins were found: 16 candidates are proteins unique to A. laidlawii, and 11 of them are surface-anchored or integral membrane proteins, which implies the presence of active signaling pathways. Among 20 acylated proteins, 14 contained palmitic chains, and six contained stearic chains. No residue of linoleic or oleic acid was observed. Acylated proteins were components of mainly sugar and inorganic ion transport systems and were surface-anchored proteins with unknown functions.