The first Japanese case of Hb Santa Ana, an unstable abnormal hemoglobin, identified rapidly by electrospray ionization mass spectrometry.
The first Japanese case of Hb Santa Ana, an unstable abnormal hemoglobin, identified rapidly by electrospray ionization mass spectrometry.
复制标题
日本首例 Hb Santa Ana 病例，这是一种不稳定的异常血红蛋白，通过电喷雾电离质谱法快速鉴定。
作者：
A. Miyazaki;T. Nakanishi;M. Kishikawa;A. Shimizu;K. Jozaki;T. Yonezawa
一个家庭的两名成员由于不稳定的血红蛋白而患有慢性溶血性贫血。经电喷雾电离质谱仪分析，在5分钟内发现了比正常β链小16u的异常β链。这一新策略也快速确定了氨基酸的取代基团。在HB Santa Ana中，血红蛋白中正常β链第88位的亮氨酸被脯氨酸取代。这是日本首次报告HB Santa Ana病例；其临床病程与先前报告的病例相似。
Two members of a family had chronic hemolytic anemia due to unstable hemoglobin. The abnormal beta-chain with a molecular weight of 16 u smaller than the normal beta-chain was found within 5 minutes by analysis using electrospray ionization mass spectrometry. The substitution of amino acid was also determined rapidly by this new strategy. The leucine at the 88th position of the normal beta-chain was substituted by proline in the hemoglobin as in Hb Santa Ana. This is the first report of a Japanese case of Hb Santa Ana; the clinical course was similar to that in the previously reported cases.
电喷雾质谱在变异血红蛋白临床诊断中的应用。
DOI：
10.1016/0378-4347(91)80576-x
发表时间：
1991
期刊：
Journal of chromatography
影响因子：
--
作者：
Shackleton,CH;Falick,AM;Green,BN;Witkowska,HE
通讯作者：
Witkowska,HE
通过组合 HPLC/电喷雾质谱法加快罕见变异血红蛋白的表征。
影响因子：
1
作者：
Witkowska,HE;Bitsch,F;Shackleton,CH
通讯作者：
Shackleton,CH