The use of MN/CA9 gene expression in identifying malignant solid renal tumors
The use of MN/CA9 gene expression in identifying malignant solid renal tumors
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DOI：
10.1016/j.eururo.2005.10.025
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发表时间：
2006-02-01
期刊：
影响因子：
23.4
通讯作者：
Tostain, J
中科院分区：
文献类型：
--
作者：
Li, GR;Cuilleron, M;Tostain, J
目的：肾脏小实体性肿瘤日益增多。明确肾实体肿瘤的恶性程度对治疗方案的选择具有重要意义。MN/CA 9在恶性肾细胞中表达，而在正常细胞中不表达。MN/CA 9是肾癌最有效的基因标记之一。本试验的目的是利用MN/CA 9基因表达的FNA活检，以确定恶性的影像不确定的实体性肾tumors.Methods：共35例影像不确定的实体性肾肿块进入本研究。分子检测方法包括快速柱提取RNA和一步法RT-PCR检测MN/CA 9基因表达。结果：28例肾透明细胞癌（透明细胞癌19例，乳头状癌7例，嫌色细胞癌2例）和7例良性肿瘤经术后病理证实。MN/CA 9的敏感性和特异性分别为68%和100%。MN/CA 9在16/19例（84%）透明细胞RCC的FNA活检中呈阳性。MN/CA 9基因表达无假阳性。假阴性或可疑细胞学检查中MN/CA 9基因表达阳性率为6 2%（8/13）。其检测有助于鉴别肾肿瘤的恶性程度。(c)2005 Elsevier B. V.保留所有权利。
Objective: Small solid renal tumors are increasingly encountered. It is important to determine the malignancy of solid renal tumors for the choice of treatment. MN/CA9 is expressed in malignant renal cells but absent in normal cells. MN/CA9 is one of the most powerful gene markers available for RCC. The objective of this pilot study is to utilize MN/CA9 gene expression in FNA biopsy to determine the malignancy of imaging-indeterminate solid renal tumors.Methods: A total of 35 patients with an imaging-indeterminate solid renal mass entered into this study. The molecular protocol consisted of a rapid column extraction of RNA and one-step RT-PCR for the detection of MN/CA9 gene expression. The preoperative molecular diagnosis was compared with postoperative pathology.Results: There were 28 RCCs (19 clear cell carcinomas, 7 papillary carcinomas and 2 chromophobe carcinomas) and 7 benign tumors proved by postoperative pathology. The overall sensitivity and specificity for MN/CA9 were respectively 68% and 100%. MN/CA9 was positive in 16/19 (84%) FNA biopsies of clear cell RCCs. No false positive appeared for MN/CA9 gene expression. Moreover, MN/CA9 gene expression was positive in 8/13 (62%) of false negative or suspect cytology.Conclusion: Detection of MN/CA9 gene expression in FNA biopsy is possible. Its detection can be helpful in identifying the malignancy among renal tumors.(c) 2005 Elsevier B.V. All rights reserved.