Phylogeography of an alpine species Primuld secundiflora inferred from the chloroplast DNA sequence variation
Phylogeography of an alpine species Primuld secundiflora inferred from the chloroplast DNA sequence variation
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从叶绿体DNA序列变异推断高山物种Primuld secundiflora的系统发育地理学
DOI：
10.3724/sp.j.1002.2008.07049
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发表时间：
2008-01
期刊：
影响因子：
--
通讯作者：
Hao, Gang
中科院分区：
文献类型：
--
作者：
Hu, Chi-Ming;Gong, Xun;Wang, Feng-Ying;Hao, Gang
横断山及其邻近地区是冰期温带植物的重要避难所。然而，它仍然是未知的HM特有种如何能够响应气候振荡。在这项研究中，我们研究了叶绿体trnL-trnF和rps 16序列的变化，报春secundiflora，一个相对常见的高山多年生植物特有的这一地区。序列数据来自11个种群的109个个体，覆盖了该物种的整个分布范围。共回收到15种单倍型，其中只有一种为3个群体所共有，其余单倍型分别固定在单个群体中。总体多样性（HT=0.966）较高，而种群内多样性（HS=0.178）较低。尽管种内形态的高度一致性，分子方差分析（AMOVA）揭示了高水平的遗传分化（97.65%）之间的群体。NST（0.982）显著高于GST（0.816）（P<0.05），呈现明显的地理分布型。单倍型的系统发育分析确定了恢复的单倍型的四个主要集群：北部的三个分支，南部的另一个分支。分支的孤立分布表明该种在冰期有多个避难所。我们没有发现这个物种的间冰期或冰后期的范围扩张，因为其他的温带植物。然而，低的种群内多样性表明，大多数种群应该经历了原地收缩-扩张周期的气候振荡。巢式进化枝分析进一步支持了这一推论，表明限制性基因流与距离隔离和异地片段化可能是塑造该物种单倍型现今空间分布的主要过程。这种特殊的地理格局可能是气候振荡和HM复杂的拓扑结构共同作用的结果。
The Hengduan Mountains (HM) and adjacent regions have been suggested as the important refugia of the temperate plants during the glacial stages. However, it remains unknown how the HM endemic species can respond to the climatic oscillations. In this study, we examined the chloroplast trnL-trnF and rps16 sequence variation of Primula secundiflora, a relatively common alpine perennial endemic to this region. Sequence data were obtained from 109 individuals of 11 populations covering the entire distribution range of the species. A total of 15 haplotypes were recovered and only one of them is commonly shared by three populations while the others are respectively fixed in the single population. The total diversity (HT=0.966) is high while the within-population diversity (HS=0.178) is low. Despite the high uniformity of the intraspecific morphology, an analysis of molecular variance (AMOVA) revealed a high level of genetic differentiation (97.65%) among populations. The higher NST (0.982) than GST (0.816) (P<0.05) suggested a distinctly phylogeographical pattern. Phylogenetic analyses of haplotypes identified four major clusters of the recovered haplotypes: three clades in the north, and the other one in the south. The isolated distribution of clades suggested multiple refugia of this species during the glacial stages. We failed to detect the interglacial or postglacial range expansion of this species as revealed for the other temper- ate plants. However, the low intra-population diversity suggested that most of the populations should have ex- perienced the in situ shrink-expansion cycles during the climatic oscillations. This inference was further supported by the nested clade analysis, which indicated that restricted gene flow with isolation by distance and allopatric fragmentation were likely the major processes that shaped the present-day spatial distribution of haplotypes in this species. Such a special phylogeographic pattern may have resulted from a combination of both climatic oscillation and complex topology of HM.
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影响因子：
16.8
作者：
A. Widmer;C. Lexer
通讯作者：
A. Widmer;C. Lexer
DOI：
10.1073/pnas.92.17.7759
发表时间：
1995-08-15
影响因子：
11.1
作者：
POWELL, W;MORGANTE, M;RAFALSKI, JA
通讯作者：
RAFALSKI, JA
DOI：
10.1007/978-3-642-83962-7
发表时间：
1991
期刊：
--
影响因子：
--
作者：
G. Hewitt;A. Johnston;J. Young
通讯作者：
G. Hewitt;A. Johnston;J. Young
影响因子：
3.3
作者：
Alan R. Templeton
通讯作者：
Alan R. Templeton
影响因子：
3.7
作者：
M. Key
通讯作者：
M. Key