Study on Dendrobium officinale O-acetyl-glucomannan (Dendronan): Part IV. Immunomodulatory activity in vivo
Study on Dendrobium officinale O-acetyl-glucomannan (Dendronan): Part IV. Immunomodulatory activity in vivo
复制标题
DOI：
10.1016/j.jff.2015.03.054
复制
发表时间：
2015-05-01
影响因子：
5.6
通讯作者：
Phillips, Glyn O.
中科院分区：
文献类型：
--
作者：
Huang, Xiaojun;Nie, Shaoping;Phillips, Glyn O.
作为一系列铁皮石斛多糖生物活性和结构研究的一部分，本研究考察了铁皮石斛及其两个多糖组分（分别为粗多糖和纯化多糖）对环磷酰胺诱导小鼠的免疫调节活性。所有这三种材料都以剂量依赖的方式保护人体免受免疫抑制。与免疫调节相关的生物活性包括刺激脾细胞增殖，平衡脾淋巴细胞亚群比例和血清细胞因子分泌，上调血清IgM、IgG和溶血素的形成，加速腹膜巨噬细胞的吞噬功能。纯化铁皮石斛多糖的调节程度高于粗多糖，表明纯化后的铁皮石斛多糖是铁皮石斛的关键生物活性成分之一，经鉴定其主要结构为o -乙酰基葡甘露聚糖（Dendronan） (C) 2015 Elsevier Ltd。版权所有。
As part of a series of studies on the bioactivities and structures of polysaccharides from Dendrobium officinale, this study investigates the immunomodulatory activities of Dendrobium officinale and its two polysaccharide fractions (crude and purified, respectively) in cyclophosphamide induced mice. All three materials protected the body from immunosuppression in a dose-dependent manner. Biological activities related to immunomodulations investigated included stimulating the proliferation of splenocytes, balancing the ratio of spleen lymphocyte subsets and the secretion of serum cytokines, up-regulating the serum IgM, IgG and haemolysin formation, and accelerating the phagocytotic function of peritoneal macrophage. The degree of modulations were higher in purified Dendrobium officinale polysaccharide than crude polysaccharide, indicating that the purified polysaccharide was one of the key bioactive components in Dendrobium officinale, the main structure of which has been identified as O-acetyl-glucomannan (Dendronan) (C) 2015 Elsevier Ltd. All rights reserved.