The Toxoplasma micropore mediates endocytosis for selective nutrient salvage from host cell compartments.
The Toxoplasma micropore mediates endocytosis for selective nutrient salvage from host cell compartments.
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DOI：
10.1038/s41467-023-36571-4
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发表时间：
2023-02-22
影响因子：
16.6
通讯作者：
Long, Shaojun
中科院分区：
文献类型：
--
作者：
Wan, Wenyan;Dong, Hui;Lai, De-Hua;Yang, Jiong;He, Kai;Tang, Xiaoyan;Liu, Qun;Hide, Geoff;Zhu, Xing-Quan;Sibley, L. David;Lun, Zhao-Rong;Long, Shaojun
Apicomplexan寄生虫的生长和复制依赖于从宿主细胞获取营养，在这种情况下，细胞内发生增殖，但营养挽救的机制仍然不清楚。大量的超微结构研究证明，在细胞内寄生虫的表面有一种质膜凹陷，有致密的颈部，称为微孔。然而，这种结构的功能仍不清楚。在这里，我们证实了在弓形虫模型中，微孔是从宿主细胞胞浆和高尔基体中摄取营养物质的必要细胞器。详细的分析表明，kelch13定位于细胞器致密的颈部，并作为微孔内摄取的蛋白质中心发挥作用。有趣的是，微孔的最大活性需要寄生虫中神经酰胺的从头合成途径。因此，这项研究为顶端复合体寄生虫获取宿主细胞来源的营养物质的潜在机制提供了洞察力，否则这些寄生虫将隔离于宿主细胞隔间。弓形虫获得宿主胞浆物质，但其机制尚不清楚。万等人的研究成果。揭示了微孔是细胞膜上内吞寄主胞质蛋白、生物素和高尔基神经酰胺的基本细胞器。
Apicomplexan parasite growth and replication relies on nutrient acquisition from host cells, in which intracellular multiplication occurs, yet the mechanisms that underlie the nutrient salvage remain elusive. Numerous ultrastructural studies have documented a plasma membrane invagination with a dense neck, termed the micropore, on the surface of intracellular parasites. However, the function of this structure remains unknown. Here we validate the micropore as an essential organelle for endocytosis of nutrients from the host cell cytosol and Golgi in the model apicomplexan Toxoplasma gondii. Detailed analyses demonstrated that Kelch13 is localized at the dense neck of the organelle and functions as a protein hub at the micropore for endocytic uptake. Intriguingly, maximal activity of the micropore requires the ceramide de novo synthesis pathway in the parasite. Thus, this study provides insights into the machinery underlying acquisition of host cell-derived nutrients by apicomplexan parasites that are otherwise sequestered from host cell compartments. Toxoplasma gondii acquires host cytosolic materials, yet the mechanism remains unknown. Wan et al. reveal the micropore as an essential organelle at the plasma membrane for endocytosis of host cytosolic proteins and biotin, and Golgi ceramide.
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影响因子：
11.4
作者：
Crawford, Michael J.;Thomsen-Zieger, Nadine;Seeber, Frank
通讯作者：
Seeber, Frank
影响因子：
11.4
作者：
Friant, S;Lombardi, R;Riezman, H
通讯作者：
Riezman, H
影响因子：
4
作者：
De Koning, HP;Al-Salabi, MI;Wastling, JM
通讯作者：
Wastling, JM
影响因子：
6.7
作者：
Gnadig, Nina F.;Stokes, Barbara H.;Fidock, David A.
通讯作者：
Fidock, David A.
DOI：
10.1016/0035-9203(63)90007-5
发表时间：
1963-01-01
影响因子：
2.2
作者：
GARNHAM, P C;BIRD, R G;BAKER, J R
通讯作者：
BAKER, J R