Imaging of lysosomal pH changes with a novel quinoline/benzothiazole probe
Imaging of lysosomal pH changes with a novel quinoline/benzothiazole probe
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使用新型喹啉/苯并噻唑探针对溶酶体 pH 变化进行成像
DOI：
10.1039/c8nj02455c
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发表时间：
2018-08-21
影响因子：
3.3
通讯作者：
Dong, Chuan
中科院分区：
文献类型：
--
作者：
Fan, Li;Nan, Ming;Dong, Chuan
合成了一种新型喹啉/苯并噻唑pH探针（E）-2-（2-（喹啉-4-基）乙烯基）苯并噻唑（BTVQ）。BTVQ对pH值有显著的响应，在3.0-3.8的极端酸度范围内，其pK（a）为3.52。此外，BTVQ显示出110 nm的显著大的斯托克斯位移，这可以减少激发干扰。BTVQ成功应用于HeLa细胞和大肠杆菌。这表明BTVQ可以作为为数不多的溶酶体特异性探针之一，用于成像酸性细胞内pH值的变化，特别是在极端酸性条件下的细菌。
A novel quinoline/benzothiazole pH probe (E)-2-(2-(quinolin-4-yl)vinyl)benzothiazole (BTVQ) was facilely synthesized. BTVQ exhibits remarkable emission response to pH and can image pH changes within the extreme acidity range of 3.0-3.8 with a pK(a) of 3.52. Moreover, BTVQ displays a notably large Stokes shift of 110 nm, which can reduce the excitation interference. BTVQ was successfully applied in HeLa cells and E. coli cells, suggesting that BTVQ can be employed as one of the few lysosome specific probes for imaging acidic intracellular pH changes, especially under extreme-acidity conditions in bacteria.