Calcium influx at the tip of growing root-hair cells of Arabidopsis thaliana
Calcium influx at the tip of growing root-hair cells of Arabidopsis thaliana
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DOI：
10.1007/bf00199963
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发表时间：
1992-07
期刊：
影响因子：
4.3
通讯作者：
J. Schiefelbein;A. Shipley;Paul Rowse
中科院分区：
文献类型：
--
作者：
J. Schiefelbein;A. Shipley;Paul Rowse
The role of extracellular Ca2+in root-hair tip growth has been investigated inArabidopsis thaliana(L.) Heynh. Root-hair length was found to be dependent on the concentration of Ca2+in the growth medium, with maximum length achieved at [Ca2+] of 0.3–3.0 mM. Using a non-intrusive calcium-specific vibrating microelectrode, an extracellular Ca2+gradient was detected at the tips of individual growing root-hair cells. The direction of the gradient indicated a net influx of Ca2+into root-hair cells. No gradient was detected near the sides of the root hairs or at the tips of non-growing root hairs. When root hairs were exposed to the Ca2+-channel blocker nifedipine, tip growth stopped and the extracellular Ca2+gradient was abolished. These results indicate that Ca2+influx through plasma-membrane Ca2+channels is required for normal root-hair tip growth.
