The mechanical stimulation of cells in 3D culture within a self-assembling peptide hydrogel
The mechanical stimulation of cells in 3D culture within a self-assembling peptide hydrogel
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DOI：
10.1016/j.biomaterials.2011.10.049
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发表时间：
2012-02-01
期刊：
影响因子：
14
通讯作者：
Naruse, Keiji
中科院分区：
文献类型：
--
作者：
Nagai, Yusuke;Yokoi, Hidenori;Naruse, Keiji
本研究的目的是提供一种支架作为一种工具，机械刺激对三维培养的细胞的影响的调查。为此，我们开发了一种人工自组装肽（SPG-178）水凝胶支架。通过衰减全反射-傅里叶变换红外光谱（ATR-FTIR）和透射电子显微镜（TEM）证实了SPG-178肽的结构特性。使用流变学测量研究SPG-178水凝胶的机械性质。SPG-178肽能够在中性pH环境中形成稳定的透明水凝胶。在SPG-178水凝胶中，小鼠骨骼肌细胞成功增殖（在8天温育期间增加12.4 +/-1.5倍;平均值+/- SEM）。当支架静态拉伸时，观察到ERK的快速磷酸化（增加2.8 +/-0.2倍;平均值+/- SEM）。这些结果表明，开发的自组装肽凝胶是无细胞毒性的，是一个合适的工具，机械刺激对三维细胞培养的影响的调查。(C)2011爱思唯尔有限公司版权所有。
The aim of this present study was to provide a scaffold as a tool for the investigation of the effect of mechanical stimulation on three-dimensionally cultured cells. For this purpose, we developed an artificial self-assembling peptide (SPG-178) hydrogel scaffold. The structural properties of the SPG-178 peptide were confirmed by attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and transmission electron microscopy (TEM). The mechanical properties of the SPG-178 hydrogel were studied using rheology measurements. The SPG-178 peptide was able to form a stable, transparent hydrogel in a neutral pH environment In the SPG-178 hydrogel, mouse skeletal muscle cells proliferated successfully (increased by 12.4 +/- 1.5 times during 8 days of incubation; mean +/- SEM). When the scaffold was statically stretched, a rapid phosphorylation of ERK was observed (increased by 2.8 +/- 0.2 times; mean +/- SEM). These results demonstrated that the developed self-assembling peptide gel is non-cytotoxic and is a suitable tool for the investigation of the effect of mechanical stimulation on three-dimensional cell culture. (C) 2011 Elsevier Ltd. All rights reserved.