Emerging Technologies to Create Inducible and Genetically Defined Porcine Cancer Models.
Emerging Technologies to Create Inducible and Genetically Defined Porcine Cancer Models.
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DOI：
10.3389/fgene.2016.00028
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发表时间：
2016
影响因子：
3.7
通讯作者：
Collares T
中科院分区：
文献类型：
--
作者：
Schook LB;Rund L;Begnini KR;Remião MH;Seixas FK;Collares T
出现了对新的动物模型的需求，以满足尚未满足的转化性癌症研究要求。转基因猪模型提供了一个特殊的机会，因为它们在遗传、解剖和生理上与人类相似。由于家畜基因组测序的最新进展和新的生物克隆技术的发展，利用猪作为一种具有与人类相似的解剖和生理特征的可塑性物种来建立癌症模型是非常可行的。本文综述了成功用于猪生物医学模型制作的基因修饰技术，特别是Cre-loxP系统，以及CRISPR/Cas9系统的主要进展和展望。猪肿瘤模型和基因组编辑方面的最新进展将把猪模型带入转化性癌症研究的前沿。
There is an emerging need for new animal models that address unmet translational cancer research requirements. Transgenic porcine models provide an exceptional opportunity due to their genetic, anatomic, and physiological similarities with humans. Due to recent advances in the sequencing of domestic animal genomes and the development of new organism cloning technologies, it is now very feasible to utilize pigs as a malleable species, with similar anatomic and physiological features with humans, in which to develop cancer models. In this review, we discuss genetic modification technologies successfully used to produce porcine biomedical models, in particular the Cre-loxP System as well as major advances and perspectives the CRISPR/Cas9 System. Recent advancements in porcine tumor modeling and genome editing will bring porcine models to the forefront of translational cancer research.