Sucrose self-administration and CNS activation in the rat
Sucrose self-administration and CNS activation in the rat
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DOI：
10.1152/ajpregu.00655.2010
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发表时间：
2011-04-01
影响因子：
2.8
通讯作者：
Zavosh, Aryana
中科院分区：
文献类型：
--
作者：
Figlewicz, Dianne P.;Bennett-Jay, Jennifer L.;Zavosh, Aryana
我们以前曾报道，胰岛素给药到下丘脑弓状核减少蔗糖的动机，评估自我管理的任务，在大鼠。由于尚未评估与蔗糖自我给药相关的中枢神经系统（CNS）激活模式，因此在本研究中，我们测量了c-Fos的表达作为神经元激活的指标。我们训练大鼠酒吧按蔗糖，根据固定比例（FR）或渐进比例（PR）的时间表和映射c-Fos的表达在中枢神经系统中的免疫反应性，与c-Fos的表达在处理控制。我们观察到一个独特的表达c-Fos在内侧下丘脑（弓状核，室旁核，视交叉后，背内侧核，腹内侧核）与PR性能的发病，和表达c-Fos在外侧下丘脑和终纹床核与FR性能的发病。FR和PR大鼠丘脑腹外侧核c-Fos表达均增加。我们的研究强调了下丘脑能量稳态回路和边缘回路在食物奖励任务执行中的重要性。考虑到内侧下丘脑在调节能量平衡中的作用，我们的研究表明，该回路可能有助于在能量稳态的更大背景下进行奖励调节。
We have previously reported that administration of insulin into the arcuate nucleus of the hypothalamus decreases motivation for sucrose, assessed by a self-administration task, in rats. Because the pattern of central nervous system (CNS) activation in association with sucrose self-administration has not been evaluated, in the present study, we measured expression of c-Fos as an index of neuronal activation. We trained rats to bar-press for sucrose, according to a fixed-ratio (FR) or progressive-ratio (PR) schedule and mapped expression of c-Fos immunoreactivity in the CNS, compared with c-Fos expression in handled controls. We observed a unique expression of c-Fos in the medial hypothalamus (the arcuate, paraventricular, retrochiasmatic, dorsomedial, and ventromedial nuclei) in association with the onset of PR performance, and expression of c-Fos in the lateral hypothalamus and the bed nucleus of stria terminalis in association with the onset of FR performance. c-Fos expression was increased in the nucleus accumbens of both FR and PR rats. Our study emphasizes the importance of both hypothalamic energy homeostasis circuitry and limbic circuitry in the performance of a food reward task. Given the role of the medial hypothalamus in regulation of energy balance, our study suggests that this circuitry may contribute to reward regulation within the larger context of energy homeostasis.