Complete Genome Sequence of a Chlorobenzene Degrader, Pandoraea pnomenusa MCB032
Complete Genome Sequence of a Chlorobenzene Degrader, Pandoraea pnomenusa MCB032
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氯苯降解剂 Pandoraea pnomenusa MCB032 的完整基因组序列
DOI：
10.1007/s00284-019-01760-2
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发表时间：
2019-11-01
影响因子：
2.6
通讯作者：
Zhou, Ning-Yi
中科院分区：
文献类型：
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作者：
Chao, Hong-Jun;Chen, Yuan-yuan;Zhou, Ning-Yi
氯苯是一种分布广泛、持久性强、毒性大的环境污染物。从功能稳定的生物反应器中分离到一株新的优势氯苯利用菌Pandoraea pnomenusa MCB 032，该菌是在Burkholderia sp. JS 150消失后，在处理氯苯过程中分离到的。本文报道了菌株MCB 032的全基因组序列，该菌株由一条环状染色体和三个质粒组成，质粒全长约6 Mb，含5450个开放阅读框，其中12个编码rRNAs，77个编码tRNAs。我们进一步确定了17个推定的基因编码的甲基接受趋化蛋白在趋化过程中感应化学梯度的酶。该菌株的注释的完整基因组序列将提供对氯代芳香族化合物降解的遗传见解。这些信息将有助于阐明生物反应器中的氯苯亲和力层次结构和物种继承。
Chlorobenzenes are ubiquitously distributed, highly persistent, and toxic environmental contaminants. Pandoraea pnomenusa MCB032 was isolated as a new dominant chlorobenzene-utilizing strain from a functionally stable bioreactor during the treatment of chlorobenzenes when strain Burkholderia sp. JS150 disappeared. In study, we report the complete genome sequence of strain MCB032 which consists of a circular chromosome and three plasmids, which are ~ 6 Mb in length with 5450 open reading frames—12 encoding rRNAs and 77 encoding tRNAs. We further identified 17 putative genes encoding the enzymes involved in the methyl-accepting chemotaxis proteins in sensing chemical gradients during chemotaxis. The annotated complete genome sequence of this strain will provide genetic insights into the degradation of chlorinated aromatic compounds. The information will empower the elucidation of chlorobenzene affinity hierarchy and species succession in the bioreactor.