Effects of altered epididymal sperm transit time on sperm quality
Effects of altered epididymal sperm transit time on sperm quality
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DOI：
10.1111/j.1365-2605.2007.00788.x
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发表时间：
2008-08-01
影响因子：
--
通讯作者：
Kempinas, Wilma De Grava
中科院分区：
文献类型：
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作者：
Fernandez, Carla Dal Bianco;Porto, Elaine Manoela;Kempinas, Wilma De Grava
附睾精子通过时间似乎在精子成熟过程中起着重要作用，而精子通过时间的改变似乎会损害精子成熟过程。本工作的目的是评价精子通过附睾的时间改变对大鼠精子参数和生育力的影响，以及睾酮在这种改变中的作用。将成年雄性SD大鼠随机分成4组，每组12天：(1)DES 10 mg/只/只/天，加速转运；(2)6.25 mg/kg/天，延迟转运；(3)与第1组相同，补充雄激素；(4)对照动物接受赋形剂。金刚乙胺治疗推迟了精子通过附睾尾的时间，刺激了该区域精子储备的增加。暴露于DES的动物表现出精子在附睾中传递时间的加快，从而降低了附睾头体部和尾部的精子密度，并降低了精子的活力。在这两种情况下，精子产生都没有改变。补充睾丸素能够将通过时间恢复到接近正常的值，因为它们高于对照组。同样的情况也发生在与精子活力有关的问题上。暴露于DES的大鼠在使用从附睾尾部收集的精子进行宫内人工授精后表现出较低的生育力。因此，可以得出结论，大鼠精子传递时间的加快似乎损害了正常的精子成熟，从而降低了精子质量和生育能力，这是一种雄激素依赖的方式。
The epididymal sperm transit time seems to have an important role in the process of sperm maturation, and it seems that alterations to the transit can harm the process. The aim of the present work was to evaluate the influence of altered sperm transit time through the epididymis on sperm parameters and fertility of rats, as well as the role of testosterone in the alterations. Sprague-Dawley adult male rats were randomly assigned to four different groups and were treated for 12 days: (i) 10 mu g/rat/day DES, to accelerate the transit; (ii) 6.25 mg/kg/day guanethidine sulphate, to delay the transit; (iii) same treatment as group 1, plus androgen supplementation; (iv) control animals received the vehicles. Guanethidine treatment delayed the sperm transit time through the epididymal cauda, provoking increased sperm reserves in this region. Animals exposed to DES showed an acceleration of sperm transit time in the epididymis, and consequently decreased sperm density in both epididymal regions, the caput-corpus and cauda, and diminished sperm motility. In both cases sperm production was not altered. Testosterone supplementation was able to restore the transit time to values close to normality, as they were higher than in the control rats. The same occurred in relation to sperm motility. Rats exposed to DES presented lower fertility after in utero artificial insemination using sperm collected from the proximal cauda epididymis. Therefore, it was concluded that the acceleration of rat sperm transit time appeared to harm normal sperm maturation, thus decreasing sperm quality and fertility capacity, in an androgen-dependent way.