Honey bee (Apis mellifera) drones survive oxidative stress due to increased tolerance instead of avoidance or repair of oxidative damage.
Honey bee (Apis mellifera) drones survive oxidative stress due to increased tolerance instead of avoidance or repair of oxidative damage.
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DOI：
10.1016/j.exger.2016.07.003
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发表时间：
2016-10
影响因子：
3.9
通讯作者：
Rueppell O
中科院分区：
文献类型：
--
作者：
Li-Byarlay H;Huang MH;Simone-Finstrom M;Strand MK;Tarpy DR;Rueppell O
氧化应激可导致过早衰老症状，并在较高剂量下导致多种生物体急性死亡。抗氧化应激性和寿命在机制和表型上是相关的；物种之间和物种内部的氧化应激抵抗力存在相当大的差异，并且通常与预期寿命相关。然而，尚不清楚抗压、长寿的个体是否会避免、修复或容忍分子损伤，从而比其他人存活得更久。蜜蜂（Apis mellifera L.）是一个新兴的模型系统，非常适合解决这个问题。此外，该物种是经济上最重要的传粉媒介，其健康可能会因接触农药（包括氧化应激源）而受到损害。在这里，我们开发了一种通过注射百草枯诱导雄性蜜蜂（雄蜂）氧化应激的方案。注射后，来自不同菌落来源的个体被置于共同的社会条件下，以监测其与注射盐水的对照组相比的生存情况。在易感和抗性个体中测量了氧化应激。百草枯大大降低了存活率，但菌落来源内部和不同菌落来源之间的个体对治疗的抵抗力各不相同。长寿的个体比早死的个体表现出更高水平的脂质过氧化。相比之下，两组之间的蛋白质羰基化水平没有显着差异。这项针对雄性蜜蜂氧化应激的首次研究表明，急性氧化应激源的存活是由于对脂质氧化损伤的耐受，而不是预防或修复。它还证明了群体在氧化应激抵抗力方面的差异，这可能有助于培育抗应激蜜蜂。
Oxidative stress can lead to premature aging symptoms and cause acute mortality at higher doses in a range of organisms. Oxidative stress resistance and longevity are mechanistically and phenotypically linked; considerable variation in oxidative stress resistance exists among and within species and typically covaries with life expectancy. However, it is unclear whether stress-resistant, long-lived individuals avoid, repair, or tolerate molecular damage to survive longer than others. The honey bee (Apis mellifera L.) is an emerging model system that is well-suited to address this question. Furthermore, this species is the most economically important pollinator, whose health may be compromised by pesticide exposure, including oxidative stressors. Here, we develop a protocol for inducing oxidative stress in honey bee males (drones) via Paraquat injection. After injection, individuals from different colony sources were kept in common social conditions to monitor their survival compared to saline-injected controls. Oxidative stress was measured in susceptible and resistant individuals. Paraquat drastically reduced survival but individuals varied in their resistance to treatment within and among colony sources. Longer-lived individuals exhibited higher levels of lipid peroxidation than individuals dying early. In contrast, the level of protein carbonylation was not significantly different between the two groups. This first study of oxidative stress in male honey bees suggests that survival of an acute oxidative stressor is due to tolerance, not prevention or repair, of oxidative damage to lipids. It also demonstrates colony differences in oxidative stress resistance that might be useful for breeding stress-resistant honey bees.