Characterization of the virE operon of the Agrobacterium Ti plasmid pTiA6.
Characterization of the virE operon of the Agrobacterium Ti plasmid pTiA6.
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DOI：
10.1093/nar/15.2.825
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发表时间：
1987-01
影响因子：
14.9
通讯作者：
S. C. Winans;P. Allenza;S. Stachel;K. Mcbride;E. Nester
中科院分区：
文献类型：
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作者：
S. C. Winans;P. Allenza;S. Stachel;K. Mcbride;E. Nester
根癌农杆菌Ti质粒含有至少六个转录单位（指定为vir基因座），其对于有效的冠瘿肿瘤发生是必需的。这些基因座之一，维雷突变，导致急剧减弱的毒力表型。在本通讯中，我们分析了维雷操纵子在分子水平上。该基因座含有编码两种亲水性蛋白质的开放阅读框，所述亲水性蛋白质具有约7，000道尔顿和60，500道尔顿的分子量。利用大肠杆菌maxicell株，在大肠杆菌中，我们已经可视化了两个由维雷编码的蛋白质，它们的大小与这些开放阅读框相对应。对维雷和其他7个vir基因座的密码子使用分析表明，与E.在大肠杆菌中，给定氨基酸的所有可能的密码子以大致相同的频率被利用。
The Agrobacterium tumefaciens Ti plasmid contains at least six transcriptional units (designated vir loci) which are essential for efficient crown gall tumorigenesis. Mutations in one of these loci, virE, result in a sharply attenuated virulence phenotype. In the present communication, we have analyzed the virE operon at the molecular level. This locus contains open reading frames coding for two hydrophilic proteins having molecular weights of approximately 7,000 daltons and 60,500 daltons. Using a maxicell strain of E. coli, we have visualized two proteins encoded by virE which correspond in size to these open reading frames. Analysis of codon usage of virE and seven other vir loci indicates that, in contrast to E. coli, all possible codons for a given amino acid are utilized at approximately the same frequency.