Timing of Gene Expression and Oolemma Localization of Mouse α6and β1Integrin Subunits during Oogenesis
Timing of Gene Expression and Oolemma Localization of Mouse α6and β1Integrin Subunits during Oogenesis
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卵子发生过程中小鼠α6和β1整合素亚基的基因表达时序和卵膜定位
DOI：
10.1006/dbio.1998.8923
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发表时间：
1998
影响因子：
2.7
通讯作者：
C. Redi
中科院分区：
文献类型：
--
作者：
M. Zuccotti;P. Rossi;E. Fiorillo;S. Garagna;A. Forabosco;C. Redi
精子抗原受精素α/β和整合素复合物α6β 1是两种最有希望的介导配子相互作用的候选蛋白。在生长过程中，当卵母细胞直径达到25-30 μm时，仓鼠和小鼠去透明带卵母细胞的质膜获得与顶体反应的精子融合的能力，这表明膜分子组成发生了变化。本研究有两个目的：（1）确定α 6和β 1整合素基因表达的时间;（2）确定这些整合素亚基在原始生殖细胞和卵母细胞中的质膜定位。我们发现α 6和β 1基因在雌性生殖细胞的所有发育阶段都有表达，从10.5到18.5 d.p.c.，在卵母细胞生长期间以及在排卵的卵中。选择性剪接亚型α 6 B从10.5 d.p.c.开始表达，而α 6A在12.5 d.p.c.开始表达，在10.5 d.p.c.，α 6或β 1的原位免疫检测显示两种整合素在雌性生殖细胞质膜上的荧光环，则荧光信号在12.5d.p.c.变得不可检测。再次出现，这次是在18.5 d.p.c.这种定位模式在从新生个体分离的卵母细胞中保持，并且只有当卵母细胞在生长期间达到直径约25-30 μm的尺寸时，荧光才会在整个卵母细胞表面周围变得均匀。这些数据虽然不是结论性的，但支持α 6和β 1整合素参与精卵融合的假说。
Abstract The sperm antigen fertilin α/β and the integrin complex α6β1present on the oolemma are two of the most promising candidates to mediate gamete interaction. During growth, the plasma membrane of both hamster and mouse zona-free oocytes acquires the capacity to fuse with acrosome-reacted sperm when oocytes reach the size of 25–30 μm in diameter, suggesting changes in the membrane molecular composition. The present study has two aims: to determine the timing of (1) gene expression of α6and β1integrins and (2) localization of these integrin subunits on the plasma membrane in primordial germ cells and in oocytes during oogenesis. We found that both α6and β1genes are expressed in female germ cells during all the stages of development analyzed, from 10.5 to 18.5 d.p.c., during oocyte growth, and in ovulated eggs. The alternatively spliced isoform α6Bis expressed from 10.5 d.p.c., whereas α6Abegins to be expressed at 12.5 d.p.c., suggesting a different role for the two variants.In situimmunodetection of α6or β1shows a ring of fluorescence on the female germ cell plasma membrane for both integrins at 10.5 d.p.c., then the fluorescent signal becomes undetectable at 12.5 d.p.c. to reappear again, this time with a patchy distribution, at 18.5 d.p.c. This pattern of localization is maintained in oocytes isolated from newborn individuals and only when oocytes during growth reach the size of about 25–30 μm in diameter does the fluorescence become homogenous all around the whole oocyte surface. These data, although not conclusive, support the hypothesis of an involvement of α6and β1integrins in sperm–egg fusion.
影响因子：
4.6
作者：
A. Sutherland;P. Calarco;C. Damsky
通讯作者：
A. Sutherland;P. Calarco;C. Damsky
胶原酶作为溶解仓鼠和小鼠卵母细胞透明带的试剂。
DOI：
10.1530/jrf.0.0930515
发表时间：
1991
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
Zuccotti,M;Urch,UA;Yanagimachi,R
通讯作者：
Yanagimachi,R
仓鼠卵膜与精子融合的能力：卵子发生过程中的获得和受精后的损失。
DOI：
10.1242/dev.112.1.143
发表时间：
1991
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Zuccotti,M;Yanagimachi,R;Yanagimachi,H
通讯作者：
Yanagimachi,H