Xenopus laevis ribosomal protein genes: isolation of recombinant cDNA clones and study of the genomic organization.
Xenopus laevis ribosomal protein genes: isolation of recombinant cDNA clones and study of the genomic organization.
复制标题
非洲爪蟾核糖体蛋白基因：重组 cDNA 克隆的分离和基因组组织的研究。
作者：
I. Bozzoni;E. Beccari;Z. Luo;F. Amaldi
来自非洲爪蟾卵母细胞的Poly-A+ mRNA，部分富集了r-蛋白编码能力，已被用作在质粒pBR 322中制备cDNA库的起始材料。已通过翻译互补mRNA选择了含有对r-蛋白特异性序列的克隆。六种不同的r-蛋白的克隆已被鉴定并用作研究其基因组组织的探针。每个单倍体基因组中发现两个基因拷贝的r-蛋白L1，L14，S19，和4 - 5蛋白S1，S8和L32。此外，已观察到含有r-蛋白S1、S8和L14序列的基因组区域的群体多态性。
Poly-A+ mRNA from Xenopus laevis oocytes, partially enriched for r-protein coding capacity has been used as starting material for preparing a cDNA bank in plasmid pBR322. The clones containing sequences specific for r-proteins have been selected by translation of the complementary mRNAs. Clones for six different r-proteins have been identified and utilized as probes for studying their genomic organization. Two gene copies per haploid genome were found for r-proteins L1, L14, S19, and four-five for protein S1, S8 and L32. Moreover a population polymorphism has been observed for the genomic regions containing sequences for r-protein S1, S8 and L14.