Alteration in lymphocyte responses, cytokine and chemokine profiles in chickens infected with genotype VII and VIII velogenic Newcastle disease virus
Alteration in lymphocyte responses, cytokine and chemokine profiles in chickens infected with genotype VII and VIII velogenic Newcastle disease virus
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DOI：
10.1016/j.cimid.2013.10.003
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发表时间：
2014-01-01
影响因子：
2
通讯作者：
Omar, Abdul Rahman
中科院分区：
文献类型：
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作者：
Rasoli, Mehdi;Yeap, Swee Keong;Omar, Abdul Rahman
新城疫（ND）是一种高度传染性禽病，是造成家禽业经济损失的主要原因之一。新城疫病毒强毒基因型的出现以及接种鸡群中新城疫病毒的反复爆发，提出了对病毒与宿主相互作用进行基础研究的必要性。在本研究中，评估了感染强效基因 VII 型新城疫病毒 IBS002 株或基因 VIII 型新城疫病毒 AF2240 株的无特定病原体 (SPF) 鸡的脾脏中 B 和 T 淋巴细胞和巨噬细胞的概况以及 26 个免疫相关基因的差异表达。感染后 1.3 和 4 天 (dpi)，在受感染的脾脏中检测到 T 淋巴细胞数量显着减少，IgM* B 细胞和 KUL01* 巨噬细胞浸润增加 (P < 0.05)。基因表达谱显示 CCLi3、CXCLi1、CXCLi2 (IL-8)、IFN-gamma、IL-12 α、IL-18、IL-1 β、IL-6、iNOS、TLR7、MHCI、IL-17F 和 TNFSF13B 上调 (P < 0.05)。然而，这两种基因型表现出不同的细胞因子表达模式和病毒载量。 IBS002 在 3 dpi 和 4 dpi 时在脾脏中显示出比 AF2240 更高的病毒载量，并在 3 dpi 时引起 CXCLi2、IFN-gamma、IL-12 α、IL-18、IL-1 β、iNOS 和 IL-10 更快的上调。同时，4 dpi 时 AF2240 中 CCLI3、CXCLi1、IFN-gamma、IL-12 α、IL-1 β 和 iNOS 基因的表达水平显着升高。此外，感染IBS002的鸡在感染后3天和4天时IL-10的表达水平显着升高。因此，强效基因型 VII 和 VIII NDV 感染诱导不同的病毒载量以及与炎症反应相关的细胞因子和趋化因子的产生。 (C) 2013 Elsevier Ltd. 保留所有权利。
Newcastle disease (ND) is a highly contagious avian disease and one of the major causes of economic losses in the poultry industry. The emergence of virulent NDV genotypes and repeated outbreaks of NDV in vaccinated chickens have raised the need for fundamental studies on the virus-host interactions. In this study, the profiles of B and T lymphocytes and macrophages and differential expression of 26 immune-related genes in the spleen of specific-pathogen-free (SPF) chickens, infected with either the velogenic genotype VII NDV strain IBS002 or the genotype VIII NDV strain AF2240, were evaluated. A significant reduction in T lymphocyte population and an increase in the infiltration of IgM* B cells and KUL01* macrophages were detected in the infected spleens at 1,3 and 4 days post-infection (dpi) (P < 0.05). The gene expression profiles showed an up-regulation of CCLi3, CXCLi1, CXCLi2 (IL-8), IFN-gamma, IL-12 alpha, IL-18, IL-1 beta, IL-6, iNOS, TLR7, MHCI, IL-17F and TNFSF13B (P < 0.05). However, these two genotypes showed different cytokine expression patterns and viral load. IBS002 showed higher viral load than AF2240 in spleen at 3 and 4 dpi and caused a more rapid up-regulation of CXCLi2, IFN-gamma, IL-12 alpha, IL-18, IL-1 beta, iNOS and IL-10 at 3 dpi. Meanwhile, the expression levels of CCLI3, CXCLi1, IFN-gamma, IL-12 alpha, IL-1 beta and iNOS genes were significantly higher in AF2240 at 4 dpi. In addition, the expression levels of IL-10 were significantly higher in the IBS002-infected chickens at 3 and 4 dpi. Hence, infection with velogenic genotype VII and VIII NDV induced different viral load and production of cytokines and chemokines associated with inflammatory reactions. (C) 2013 Elsevier Ltd. All rights reserved.