Anti-ulcer effect of tea catechin in rats
Anti-ulcer effect of tea catechin in rats
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DOI：
10.1248/bpb.29.2206
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发表时间：
2006-11-01
影响因子：
2
通讯作者：
Ito, Mikio
中科院分区：
文献类型：
--
作者：
Hamaishi, Kanoko;Kojima, Ryoji;Ito, Mikio
口服儿茶素剂量依赖性地防止无水乙醇（50，100，200 mg/kg）或束缚加水浸应激（300，400 mg/kg）诱导的大鼠急性胃粘膜损伤。当在对大鼠注射乙酸后第15天评价测试化合物的效果时，重复口服给予茶儿茶素（25、50、100 mg/kg，每日两次）剂量依赖性地加速乙酸诱导的慢性胃溃疡的愈合。儿茶素（10（-5）~ 40（-1）g/100 ml）对超氧阴离子的清除作用呈浓度依赖性。茶儿茶素（100，200毫克/公斤口服）显着抑制硫代巴比妥酸反应性物质的增加，在大鼠损伤的粘膜50%乙醇处理。茶儿茶素（50、100 mg/kg，每日两次口服）在第7天和第15天明显抑制醋酸诱导胃溃疡溃疡区域硫代巴比妥酸反应物质含量的增加。此外，在50、100和200 mg/kg经口给药时，其剂量依赖性地防止了无水乙醇诱导的胃粘膜氨基己糖含量降低，但未能抑制基础胃酸分泌。提示茶儿茶素可能主要通过其抗氧化和增加胃粘液的作用对急性胃粘膜损伤起到保护作用，并促进慢性胃溃疡的愈合。
Oral administration of tea catechin dose-dependently prevented absolute ethanol-induced (50, 100, 200 mg/kg) or restraint plus water immersion stress-induced acute gastric mucosal injury (300, 400 mg/kg) in rats. When the effect of test compound was evaluated on the 15th day after acetic acid injection to rats, repeated oral administration of tea catechin (25, 50, 100 mg/kg twice daily) dose-dependently accelerated the healing of acetic acid-induced chronic gastric ulcers. Tea catechin (10(-5)-40(-1) g/100 ml) concentration-dependently scavenged superoxide anions in vitro. Tea catechin (100, 200 mg/kg orally) markedly inhibited the increase in thiobarbituric acid-reactive substances in the injured mucosa of rats treated with 50% ethanol. Tea catechin (50, 100 mg/kg twice orally, daily) markedly inhibited the increase in content of thiobarbituric acid-reactive substances in the ulcerated region of acetic acid-induced gastric ulcers on the 7th and 15th days. In addition, at 50, 100 and 200 mg/kg orally, it dose-dependently prevented the decrease in gastric mucosal hexosamine content induced by absolute ethanol, although it failed to inhibit the basal gastric acid secretion. These results suggest that tea catechin may primarily protect gastric mucosa from acute gastric mucosal injury and promote the healing of chronic gastric ulcers by its antioxidant activity and gastric mucus-increasing actions.