Synthesis, characterization, and utility of thermoresponsive natural/unnatural product macroligands for affinity chromatography.
Synthesis, characterization, and utility of thermoresponsive natural/unnatural product macroligands for affinity chromatography.
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用于亲和色谱的热响应性天然/非天然产物大配体的合成、表征和效用。
DOI：
10.1021/ol062045w
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发表时间：
2006
期刊：
影响因子：
5.2
通讯作者：
Romo,Daniel
中科院分区：
文献类型：
--
作者：
Zhou,Min;Sivaramakrishnan,Ananthapadmanab;Ponnamperuma,Krishan;Low,Woon-Kai;Li,Chunmei;Liu,JunO;Bergbreiter,DavidE;Romo,Daniel
描述了用作新型亲和树脂的、具有环孢菌素 A (CsA) 和地塞米松 (Dex) 的热响应性水溶性聚 N-异丙基丙烯酰胺 (PNIPAM) 衍生大配体的合成和表征。这些可溶性大配体的表征，包括配体负载和完整性，通过 1 H NMR 光谱测定。其中一种 CsA 大配体用于蛋白质亲和实验，从 Jurkat T 细胞裂解物中捕获已知的 CsA 结合蛋白（亲环蛋白）。
The synthesis and characterization of thermoresponsive, water-soluble poly-N-isopropyl acrylamide (PNIPAM) derived macroligands displaying cyclosporin A (CsA) and dexamethasone (Dex) for use as novel affinity resins are described. Characterization of these soluble macroligands, including ligand loading and integrity, was determined by1H NMR spectroscopy. One of the CsA macroligands was used in a protein affinity experiment to capture known binding proteins of CsA, the cyclophilins, from Jurkat T-cell lysates.