BY4741 cannot enter quiescence from rich medium.
BY4741 cannot enter quiescence from rich medium.
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DOI：
10.17912/micropub.biology.000742
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发表时间：
2023
影响因子：
--
通讯作者：
Breeden, Linda
中科院分区：
文献类型：
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作者：
Miles, Shawna;Lee, Cameron;Breeden, Linda
在丰富的培养基中，W303酿酒酵母在耗尽可用葡萄糖之前一小时开始在G1中积累。它经历了一次不对称的细胞分裂，然后在G1期停止分裂。相比之下，BY4741在葡萄糖耗尽前四小时停止分裂，细胞密度为W303的四分之一。没有不对称的细胞分裂，只有50%的细胞停滞在G1期。我们得出结论，BY4741的生长不受葡萄糖的限制，并且当它们从富培养基进入静止期时，它们不经历由其他菌株进行的常规事件。在W303中，葡萄糖限制和向静止过渡的时间与生物量积累速率和细胞倍增时间相关。
In rich medium, W303 Saccharomyces cerevisiae begins to accumulate in G1 an hour before it exhausts the available glucose. It undergoes one more asymmetrical cell division, then stops dividing in G1. In contrast, BY4741, stops dividing four hours before glucose exhaustion, at one-fourth the cell density achieved by W303. There is no asymmetrical cell division and only 50% of the cells arrest in G1. We conclude that BY4741 growth is not limited by glucose and they do not go through the stereotypical events carried out by other strains as they enter quiescence from rich medium. In W303, the timing of glucose limitation and the transition to quiescence is correlated with the rate of biomass accumulation and cell doubling time.