DNA separation with low-viscosity sieving matrix on microfabricated polycarbonate microfluidic chips
DNA separation with low-viscosity sieving matrix on microfabricated polycarbonate microfluidic chips
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DOI：
10.1007/s00216-004-2988-0
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发表时间：
2005-02-01
影响因子：
4.3
通讯作者：
Fang, ZL
中科院分区：
文献类型：
--
作者：
Ye, MY;Yin, XF;Fang, ZL
微流体装置已制造聚碳酸酯（PC）基板上通过使用热压方法，使用硅主模板。通过在硅母板上的功能通道周围添加辅助线，显著延长了其使用寿命，并大大提高了PC盖板与微流控芯片的结合强度。300多个聚碳酸酯微流控芯片已经用相同的硅模具复制。Phi X-174/HaeIII DNA限制性片段的CE分离，具有高分辨率和良好的重现性，在这些设备上使用低粘度筛分基质HPMC-50实现。发现温度对分离效率有显著影响。
Microfluidic devices have been fabricated on polycarbonate (PC) substrates by use of a hot embossing method using a silicon master template. By adding auxiliary lines around the functional channel on the silicon master, its lifetime was significantly prolonged and the bonding strength of the PC cover plate to the microfluidic chip was greatly improved. More than 300 polycarbonate microfluidic chips have been replicated with the same silicon mold. CE separation of Phi X-174/HaeIII DNA restriction fragments, with high resolution efficiency and good reproducibility, was achieved on these devices using the low-viscosity sieving matrix HPMC-50. Temperature was found to have a significant effect on separation efficiency.