MICROINJECTION OF P34CDC2 KINASE INDUCES MARKED CHANGES IN CELL-SHAPE, CYTOSKELETAL ORGANIZATION, AND CHROMATIN STRUCTURE IN MAMMALIAN FIBROBLASTS
MICROINJECTION OF P34CDC2 KINASE INDUCES MARKED CHANGES IN CELL-SHAPE, CYTOSKELETAL ORGANIZATION, AND CHROMATIN STRUCTURE IN MAMMALIAN FIBROBLASTS
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DOI：
10.1016/0092-8674(90)90725-t
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发表时间：
1990-01-12
期刊：
影响因子：
64.5
通讯作者：
CAVADORE, JC
中科院分区：
文献类型：
--
作者：
LAMB, NJC;FERNANDEZ, A;CAVADORE, JC
我们已经研究了提高细胞内p34 cdc 2激酶水平的影响，通过显微注射到活的哺乳动物细胞。这些研究揭示了细胞形状的快速和戏剧性的变化，细胞变圆并失去了大部分细胞-基质接触。这种作用在细胞周期中除了S期外的所有时间都被诱导，并且在S期或有丝分裂中是完全可逆的。用p34 cdc 2激酶或与细胞周期蛋白B相关的p34 cdc 2激酶的均相催化亚基也得到了类似的结果。这些变化伴随着一个显着减少间期微管没有纺锤体的形成，肌动蛋白微丝重新分布，和过早的染色质凝聚。虽然这些变化密切模仿有丝分裂的早期阶段发生的事件，p34 cdc 2激酶注射的细胞没有被诱导通过进一步分裂。这些数据提供了详细的证据表明，p34 cdc 2激酶在与哺乳动物细胞有丝分裂相关的细胞结构重排中起着重要的先决条件作用。
We have examined the effects of elevating the intracellular levels of p34cdc2 kinase by microinjection into living mammalian cells. These studies reveal rapid and dramatic changes in cell shape with cells becoming round and losing the bulk of their cell-substratum contact. Such effects were induced at all times in the cell cycle except at S phase and were fully reversible at S phase or mitosis. Similar results were obtained with the homogeneous catalytic subunit of p34cdc2 kinase or p34cdc2 kinase associated with cyclin B. These alterations were accompanied by a marked reduction in interphase microtubules without the spindle formation, actin microfilament redistribution, and premature chromatin condensation. Although these changes closely mimic the events occurring during early phases of mitosis, p34cdc2 kinase-injected cells were not induced to pass further into division. These data provide detailed evidence that p34cdc2 kinase plays a major prerequisite role in the rearrangement of cellular structures associated with mammalian cell mitosis.