Biological properties and spectrum of activity of Pseudomonas syringae pv syringae toxins
Biological properties and spectrum of activity of Pseudomonas syringae pv syringae toxins
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DOI：
10.1006/pmpp.1996.0078
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发表时间：
1997-02-01
影响因子：
2.7
通讯作者：
Graniti, A
中科院分区：
文献类型：
--
作者：
Lavermicocca, P;Iacobellis, NS;Graniti, A
丁香霉素E（SR-E）、丁香毒素（ST）和丁香肽素（SP）的两种形式（SP22-A和SP25-A）是由假单胞菌pv.对它们的生物活性进行了研究。所有毒素引起绵羊红细胞裂解，但SP比SR-E和ST更活跃。裂解浓度分别为0.8和0.6 μ M的SP22-A和SP25-A和1.4和5.9 μ M的SR-E和ST，分别。毒素对胡萝卜组织电解质渗漏和马铃薯块茎组织活力的影响证实了SP的植物毒性高于SR-E或ST。0.4 μ M SP22-A和10 μ M ST分别诱导了对电解质渗漏的相关影响。抗菌活性谱表明，50 μ M毒素溶液不抑制革兰氏阴性菌的生长。革兰氏阳性菌对SPs敏感，而SR-E和ST对这些细菌的生长无明显影响。特别是，巨大芽孢杆菌的生长被1.56 μ M的SP22-A和3.12 μ M的SP25-A抑制，但不受50 μ M的SR-E或ST。毒素对真菌的影响是可变的。例如，6.25 μ M SR-E或ST、12.5 μ M SP22-A和37.5 μ M SP25-A抑制酵母毛红酵母的生长。1.6 μ M的SP25-A、12.5 μ M的SP22-A、18.7 μ M的SR-E和25 μ M的ST对植物病原真菌灰葡萄孢（Botrytis cinerea）的菌丝生长均有抑制作用，其中SR-E对灰葡萄孢孢子萌发和芽管伸长的抑制作用最强。(C)出版社：Academic Press Limited。
Syringomycin E (SR-E), syringotoxin (ST) and two forms (SP22-A and SP25-A) of syringopeptin (SP), the main lipodepsipeptides produced by strains of Pseudomonas syringae pv. syringae, were investigated for their biological activities. All toxins caused lysis of sheep erythrocytes, but SPs were more active than SR-E and ST. The lytic concentrations were 0.8 and 0.6 mu m for SP22-A and SP25-A and 1.4 and 5.9 mu M for SR-E and ST, respectively. The effect of toxins on the electrolyte leakage from carrot tissues and on the viability of potato tuber tissues confirmed the higher phytotoxicity of SPs in comparison to the SR-E or ST. A relevant effect on electrolyte leakage was induced by 0.4 mu M SP22-A and 10 mu M ST, respectively. The spectrum of antimicrobial activity indicated that 50 mu M toxin solutions did not inhibit the growth of Gram-negative bacteria. Grampositive bacteria were sensitive to SPs but in contrast, SR-E and ST had no relevant effect on the growth of these bacteria. In particular, the growth of Bacillus megaterium was inhibited by 1.56 mu M SP22-A and 3.12 mu M SP25-A, but was unaffected by 50 mu M SR-E or ST. The effects of toxins on fungi were variable. For example, 6.25 mu M SR-E or ST, 12.5 mu M SP22-A and 37.5 mu M SP25-A inhibited the growth of the yeast Rhodotorula pilimanae. The mycelial growth of the plant pathogenic fungus Botrytis cinerea was inhibited by 1.6 mu M SP25-A, 12.5 mu M SP22-A, 18.7 mu M SR-E and 25 mu M ST. Furthermore, SR-E was the most active toxin in inhibiting conidial germination and germ tube elongation of this fungus. (C) 1997 Academic Press Limited.