DNA sequence preference of the progesterone receptor.
DNA sequence preference of the progesterone receptor.
复制标题
黄体酮受体的 DNA 序列偏好。
DOI：
10.1073/pnas.80.1.16
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发表时间：
1983
影响因子：
11.1
通讯作者：
O'Malley,BW
中科院分区：
文献类型：
--
作者：
Compton,JG;Schrader,WT;O'Malley,BW
高纯度的母鸡输卵管孕酮受体A亚基与多个鸡基因DNA结合。硝酸纤维素膜吸附从重组质粒中获得的[32P]DNA片段的序列偏好显示，某些DNA片段有明显的保留。类固醇调节基因卵清蛋白和Y基因5‘端的DNA片段有约10倍的偏好，鸡β-珠蛋白和α-肌动蛋白基因的类似DNA片段没有偏好。限制性内切酶图谱显示卵清蛋白基因5‘端存在多个受体相互作用位点。其中一个优先结合位点位于转录起始上游的-135至-247个碱基对之间。这个区域包含一个18碱基对的富含A+T的序列，很可能是结合位点本身的候选序列，因为早期的研究表明受体A对富含A+T的DNA有明显的偏好。
Highly purified hen oviduct progesterone receptor A subunit was tested for binding to several chicken gene DNAs. Sequence preference detected by nitrocellulose filter adsorption of [32P]DNA fragments obtained from recombinant plasmids revealed a marked retention of certain DNA fragments. About a 10-fold preference was seen for DNA fragments flanking the 5' end of the steroid-regulated genes ovalbumin and gene Y. No preference was seen with analogous DNA fragments from chicken beta-globin and alpha-actin genes. Restriction endonuclease mapping suggests the presence of multiple receptor interaction sites flanking the 5' terminus of the ovalbumin gene. One of these preferential binding sites was localized between -135 and -247 base pairs upstream from the start of transcription. This region contains an 18-base-pair A + T-rich sequence, a likely candidate for the binding site itself, because earlier studies had shown receptor A to have marked preference for A + T-rich DNA.