Exploration of key regulators driving primary feather follicle induction in goose skin
Exploration of key regulators driving primary feather follicle induction in goose skin
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DOI：
10.1016/j.gene.2020.144338
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发表时间：
2020-03-20
期刊：
影响因子：
3.5
通讯作者：
Mou, Chunyan
中科院分区：
文献类型：
--
作者：
Hu, Xuewen;Zhang, Xiaokang;Mou, Chunyan
初级毛囊是广泛分布于羽毛鸟类皮肤中的一种普遍的皮肤附属物。鹅皮初级毛囊的形态发生和发育在很大程度上尚不清楚。本研究通过新转录组分析，研究了鹅胚胎皮肤初级毛囊的诱导(诱导前和诱导前)，发现了409个差异表达基因(DEG)。DIGs可能分别调节由胎盘(4个基因)和真皮凝集物(12个基因)组成的毛囊原基的从头形成，以及表皮(5个基因)和真皮成纤维细胞(17个基因)的增厚。进一步分析丰富的DEGS为细胞黏附和KEGG通路，包括与细胞通讯和分子调控高度相关的Wnt和Hedgehog信号通路。通过定量聚合酶链式反应检测到6个Wnt途径基因在鹅皮初级毛囊诱导过程中表达上调。原位杂交结果显示，WNT16、SFRP1和FRZB在初级羽毛原基中表达较弱，而FZD1、LEF1和Dkk1最初在毛囊间皮肤和毛囊原基中表达，然后主要局限于羽毛原基。时空表达谱表明，Wnt通路基因Dkk1、FZD1和LEF1是鹅皮初级毛囊诱导过程中的重要调控基因。本报告揭示的动态分子变化和特定的基因表达模式提供了水禽初级羽毛毛囊和皮肤发育的一般知识，并有助于进一步了解小鼠和鸡模型以外的毛发和羽毛发育的多样性。
The primary feather follicles are universal skin appendages widely distributed in the skin of feathered birds. The morphogenesis and development of the primary feather follicles in goose skin remain largely unknown. Here, the induction of primary feather follicles in goose embryonic skin (pre-induction vs induction) was investigated by de novo transcriptome analyses to reveal 409 differentially expressed genes (DEGs). The DEGs were characterized to potentially regulate the de novo formation of feather follicle primordia consisting of placode (4 genes) and dermal condensate (12 genes), and the thickening of epidermis (5 genes) and dermal fibroblasts (17 genes), respectively. Further analyses enriched DEGs into GO terms represented as cell adhesion and KEGG pathways including Wnt and Hedgehog signaling pathways that are highly correlated with cell communication and molecular regulation. Six selected Wnt pathway genes were detected by qPCR with up-regulation in goose skin during the induction of primary feather follicles. The localization of WNT16, SFRP1 and FRZB by in situ hybridization showed weak expression in the primary feather primordia, whereas FZD1, LEF1 and DKK1 were expressed initially in the inter-follicular skin and feather follicle primordia, then mainly restricted in the feather primordia. The spatial-temporal expression patterns indicate that Wnt pathway genes DKK1, FZD1 and LEF1 are the important regulators functioned in the induction of primary feather follicle in goose skin. The dynamic molecular changes and specific gene expression patterns revealed in this report provide the general knowledge of primary feather follicle and skin development in waterfowl, and contribute to further understand the diversity of hair and feather development beyond the mouse and chicken models.