Hepatitis B virus infection in lymphatic tissues in inactive hepatitis B carriers
Hepatitis B virus infection in lymphatic tissues in inactive hepatitis B carriers
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DOI：
10.1016/j.jhep.2005.01.016
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发表时间：
2005-06-01
影响因子：
25.7
通讯作者：
Chiba, T
中科院分区：
文献类型：
--
作者：
Umeda, M;Marusawa, H;Chiba, T
背景/目的：肝外组织中的B型肝炎病毒（HBV）感染是有争议的。为了阐明游离型HBV是否可以感染非肝组织，我们研究了无病毒血症的非活动性HBV携带者淋巴细胞中HBV的分子形式，从而避免了来自血清中病毒颗粒的HBV基因组的污染。方法：我们评估了HBV基因组、复制形式和肝脏、血清、外周血单核细胞（PBMC）中的病毒整合体，结果：21例抗-HBc阳性者中，15例（71.4%）肝组织、11例（52.4%）淋巴结和3例（14.3%）PBMC中检出HBV-DNA。然而，从淋巴组织中检测到的HBV基因组均不包括HBV的复制形式。1例患者淋巴组织中存在整合型HBV，17号染色体内含子序列中存在宿主-病毒连接。结论：抗-HBc阳性的HBV携带者淋巴组织不能支持病毒复制，但病毒基因组仍以整合形式存在。(C)2005年欧洲肝脏研究协会。Elsevier B. V.出版，保留所有权利。
Background/Aims: Hepatitis B virus (HBV) infection in extrahepatic tissues is controversial. To clarify whether episomal HBV can infect nonhepatic tissues, we investigated the molecular forms of HBV in the lymphatic cells of inactive HBV carriers who lacked viremia, thus avoiding contamination with HBV genomes originating from the viral particles present in the serum.Methods: We assessed HBV genome, replicative forms, and viral integrants in the liver, serum, peripheral blood mononuclear cells (PBMC), and lymph nodes of 21 inactive HBV carriers who tested positive for antibodies against the HBV core antigen (anti-HBc).Results: Of the 21 anti-HBc positive individuals, HBV-DNA was detected in liver samples of 15 (71.4%), in the lymph nodes of 11 (52.4%), and in PBMC of three (14.3%). However, none of the detected HBV genomes from lymphatic tissues included the replicative forms of HBV. In one case, integrated HBV was present in the lymphatic tissues and the host-viral junction was present in the intronic sequences of chromosome 17.Conclusions: These data suggest that human lymphatic tissues cannot support viral replication in anti-HBc positive inactive HBV carriers, while retaining the viral genome as an integrated form. (C) 2005 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.