The reduction of DSS-induced colitis severity in mice exposed to cigarette smoke is linked to immune modulation and microbial shifts
The reduction of DSS-induced colitis severity in mice exposed to cigarette smoke is linked to immune modulation and microbial shifts
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在暴露于香烟烟雾的小鼠中，dss诱导的结肠炎严重程度的降低与免疫调节和微生物转移有关
DOI：
10.1038/s41598-020-60175-3
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发表时间：
2020-03-02
影响因子：
4.6
通讯作者：
Hoeng, Julia
中科院分区：
文献类型：
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作者：
Lo Sasso, Giuseppe;Phillips, Blaine W.;Hoeng, Julia
接触香烟烟雾会对健康造成有害影响，增加接触者患心血管、肺部疾病和致癌的风险。CS对炎症性肠病（IBD）的影响已被许多流行病学和临床研究所证实。事实上，CS与发生克罗恩病（CD）的高风险相关，而与溃疡性结肠炎（UC）的发展、疾病风险和复发率呈负相关。为了研究CS暴露对实验性结肠炎的影响，我们对暴露于葡聚糖硫酸钠（DSS）和CS的小鼠结肠转录组和微生物组进行了全面综合的比较分析。结肠转录组分析显示，CS以浓度依赖的方式下调特定途径，影响肠道微生物组的炎症状态和组成。宏基因组学分析表明，CS可以调节dss诱导的特定细菌属的生态失调，有助于解决炎症或加速恢复。吸烟的风险远远大于任何可能的好处，因此必须始终鼓励戒烟，因为它对健康有重大好处。然而，主动吸烟与UC发展之间的负相关关系不容忽视，本研究为研究与燃烧分离的烟草中某些化合物衰减结肠炎的潜在分子机制奠定了基础。
Exposure to cigarette smoke (CS) causes detrimental health effects, increasing the risk of cardiovascular, pulmonary diseases and carcinogenesis in exposed individuals. The impact of CS on Inflammatory Bowel Disease (IBD) has been established by a number of epidemiological and clinical studies. In fact, CS is associated with a higher risk of developing Crohn's disease (CD) while inversely correlates with the development, disease risks, and relapse rate of ulcerative colitis (UC). To investigate the effect of CS exposure on experimental colitis, we performed a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS. Colon transcriptome analysis revealed that CS downregulated specific pathways in a concentration-dependent manner, affecting both the inflammatory state and composition of the gut microbiome. Metagenomics analysis demonstrated that CS can modulate DSS-induced dysbiosis of specific bacterial genera, contributing to resolve the inflammation or accelerate recovery. The risks of smoking far outweigh any possible benefit, thus smoking cessation must always be encouraged because of its significant health benefits. However, the inverse association between active smoking and the development of UC cannot be ignored and the present study lays the foundation for investigating potential molecular mechanisms responsible for the attenuation of colitis by certain compounds of tobacco when decoupled from combustion.