High susceptibility of obese hypertensive SHRSP.Z-Lepr(fa) /IzmDmcr rats to lipid deposition in the mesenteric artery
High susceptibility of obese hypertensive SHRSP.Z-Lepr(fa) /IzmDmcr rats to lipid deposition in the mesenteric artery
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肥胖高血压SHRSP.Z-Lepr(fa)/IzmDmcr大鼠对肠系膜动脉脂质沉积的高度易感性
DOI：
10.1111/j.1440-1681.2010.05440.x
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Mori M
中科院分区：
文献类型：
--
作者：
Kunimasa K;Yoshitomi H;Miura C;Mori H;Tsuchikura S;Ikeda K;Gao M;Yamori Y;Mori M
1. 动脉粥样硬化常见于肥胖。肥胖动脉粥样硬化易感动物可能是了解肥胖相关动脉粥样硬化病理生理学的一个有前途的工具。然而，大多数大鼠品系对动脉粥样硬化有抵抗力。本研究的目的是评估两种肥胖高血压大鼠模型，即SHRSP的易感性。Z‐Leprfa/IzmDmcr大鼠（SHRSP‐fatty）和SHR。通过将这些品系与非肥胖卒中易感性自发性高血压大鼠（SHRSP）进行比较，发现Cg‐Leprcp/NDmcr大鼠（SHR‐cp）与动脉脂质沉积（动脉粥样硬化的初始阶段）之间存在显著差异。8周龄的雄性SHRSP、SHRSP - fatty和SHR - cp被喂食含有20%棕榈油、5%胆固醇和2%胆酸的高脂肪和高胆固醇饮食，为期5周。在12周龄大鼠中测量体重、血压和空腹血清总胆固醇和甘油三酯水平。油红O染色显示肠系膜动脉内脂质沉积。12周龄SHRSP - fatty和SHR - cp的体重高于SHRSP （P< 0.005）。SHRSP组和SHRSP‐fatty组的收缩压高于SHR‐cp组（P< 0.005）。与SHRSP组相比，SHRSP - fatty组血清总胆固醇和甘油三酯水平升高（P< 0.005）， SHR - cp水平升高（P< 0.05）。SHRSP‐脂肪组的肠系膜动脉脂质沉积明显高于SHRSP组（分别为37.7±4.9vs13.1±2.8%，P< 0.005），而SHR‐cp组的脂质沉积明显减少（1.8±0.4%，P< 0.05）。本研究结果表明，SHRSP‐fatty对动脉脂质沉积高度敏感，而SHR‐cp则具有抗性。因此，SHRSP‐fatty可能是一种有用的肥胖大鼠模型，用于研究动脉粥样硬化过程。
1. Atherosclerosis is commonly observed in obesity. Obese atherosclerosis‐prone animals may be a promising tool for understanding the pathophysiology of obesity‐associated atherosclerosis. However, most rat strains are resistant to atherosclerosis. The aim of the present study was to assess the susceptibility of two obese hypertensive rat models, namely SHRSP.Z‐Leprfa/IzmDmcr rats (SHRSP‐fatty) and SHR.Cg‐Leprcp/NDmcr rats (SHR‐cp), to arterial lipid deposition, an initial stage of atherosclerosis, by comparing these strains with non‐obese stroke‐prone spontaneously hypertensive rats (SHRSP).2. Eight‐week‐old male SHRSP, SHRSP‐fatty and SHR‐cp were fed a high‐fat and high‐cholesterol diet containing 20% palm oil, 5% cholesterol and 2% cholic acid for 5 weeks. Bodyweight, blood pressure and fasting serum levels of total cholesterol and triglycerides were measured in 12‐week‐old rats. Oil red O staining was used to visualize lipid deposition in the mesenteric artery.3. The bodyweight of 12‐week‐old SHRSP‐fatty and SHR‐cp was higher than that of SHRSP (P< 0.005). Systolic blood pressure in SHRSP and SHRSP‐fatty was higher than in SHR‐cp (P< 0.005). Serum total cholesterol and triglyceride levels were elevated in SHRSP‐fatty (P< 0.005) and SHR‐cp (P< 0.05) compared with levels in SHRSP. Lipid deposition in the mesenteric artery was significantly greater in SHRSP‐fatty than in SHRSP (37.7 ± 4.9vs13.1 ± 2.8%, respectively;P< 0.005), but markedly reduced in SHR‐cp (1.8 ± 0.4%;P< 0.05).4. The results of the present study indicate that SHRSP‐fatty are highly susceptible to arterial lipid deposition, whereas SHR‐cp are resistant. Thus, SHRSP‐fatty may be a useful obese rat model in which to investigate atherosclerotic processes.