Simple and broadly applicable sample preparation by use of magnetic glass particles
Simple and broadly applicable sample preparation by use of magnetic glass particles
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DOI：
10.1515/cclm.1998.095
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发表时间：
1998-08-01
影响因子：
6.8
通讯作者：
Kleiber, J
中科院分区：
文献类型：
--
作者：
Bartl, K;Wenzig, P;Kleiber, J
与传染病检测和基因检测相关的核酸检测的一个关键问题是样品制备。这必须方便、高效且适合自动化。我们描述了一种基于使用玻璃磁性颗粒的新方法。所涉及的步骤是通过离液盐裂解细菌细胞和病毒颗粒、将释放的核酸与磁性玻璃颗粒结合、去除抑制剂以及释放准备用于PCR扩增的纯化核酸。该方法满足样品制备的大部分要求，例如灵敏度、通用性和自动化适用性。
A key issue in nucleic acid-based assays related to infectious disease testing and genetic testing is the sample preparation. This has to be convenient, efficient and suitable for automation.We describe a new method based on the use of glass magnetic particles. The steps involved are the lysis of bacterial cells and viral particles by chaotropic salts, binding of released nucleic acids to the magnetic glass particles, the removal of inhibitors and the release of purified nucleic acids ready for PCR amplification. The method fulfills most of the requirements for sample preparation such as sensitivity, universality and suitability for automation.