MabZIP74 interacts with MaMAPK11-3 to regulate the transcription of MaACO1/4 during banana fruit ripening
MabZIP74 interacts with MaMAPK11-3 to regulate the transcription of MaACO1/4 during banana fruit ripening
复制标题
MabZIP74 与 MaMAPK11-3 相互作用，调节香蕉果实成熟过程中 MaACO1/4 的转录
DOI：
10.1016/j.postharvbio.2020.111293
复制
发表时间：
2020-11-01
影响因子：
7
通讯作者：
Kuang, Jian-fei
中科院分区：
文献类型：
--
作者：
Liang, Shu-min;Chen, Si-cen;Kuang, Jian-fei
果实成熟是许多可食用水果的重要经济过程，其由协调大量基因和/或蛋白质的时空表达的多水平调控网络控制。在香蕉中，乙烯协调成熟过程，但控制编码乙烯生物合成相关酶的基因表达的调控机制尚未完全了解。在这里，我们报告的基本亮氨酸拉链（bZIP）转录因子MabZIP 74从香蕉果实的鉴定和表征。随着香蕉果实成熟进程的进行，MabZIP 74的表达逐渐降低，表明MabZIP 74在香蕉果实成熟中起负作用。MabZIP 74位于细胞核中并显示转录抑制活性。更具体地说，MabZIP 74被发现是乙烯生物合成基因MaACO 1和MaACO 4的转录抑制因子，其积累抑制香蕉成熟期间的乙烯产生。重要的是，蛋白激酶MaMAPK 11 -3与MabZIP 74相互作用并磷酸化，这减弱了成熟过程中MabZIP 74介导的MaACO 1和MaACO 4的转录抑制。总之，我们的研究结果揭示了一种新的MabZIP 74介导的调控网络参与乙烯生物合成和香蕉果实成熟。这些结果推进了我们对果实成熟的认识，并为香蕉果实成熟的遗传改良提供了额外的机会。
Fruit ripening is an economically important process of many edible fruits, which is controlled by a multi-level regulatory network coordinating the spatio-temporal expression of a large array of genes and/or proteins. In banana, ethylene orchestrates the ripening process, but the regulatory mechanism(s) controlling the expression of genes encoding ethylene biosynthesis-related enzymes is not fully understood. Here, we report the identification and characterization of a basic leucine zipper (bZIP) transcription factor MabZIP74 from banana fruit. Its gradually decreased with the progression of banana fruit ripening, implying a negative role for MabZIP74 in banana fruit ripening. MabZIP74 is localized in the nucleus and displays transcriptional inhibitory activity. More specifically, MabZIP74 was found to be a transcriptional repressor of ethylene biosynthetic genes MaACO1 and MaACO4, the accumulation of which paralleled ethylene production during banana ripening. Importantly, a protein kinase MaMAPK11-3 interacted with and phosphorylated MabZIP74, which attenuated MabZIP74-mediated transcriptional repression of MaACO1 and MaACO4 during ripening. Taken together, our findings reveal a novel MabZIP74-mediated regulatory network involved in ethylene biosynthesis and fruit ripening in banana. These results advance our knowledge on fruit ripening and provide additional opportunities for genetic improvement of banana fruit ripening.