Encapsulation of Lactobacillus plantarum 423 and its Bacteriocin in Nanofibers
Encapsulation of Lactobacillus plantarum 423 and its Bacteriocin in Nanofibers
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DOI：
10.1007/s12602-009-9024-9
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发表时间：
2010-03-01
影响因子：
4.9
通讯作者：
Dicks, L. M. T.
中科院分区：
文献类型：
--
作者：
Heunis, T. D. J.;Botes, M.;Dicks, L. M. T.
植物乳杆菌423产生的植物抗菌素423被包裹在由18%(w/v)聚氧乙烷(200000 Da)静电纺丝而成的纳米纤维中。纳米纤维的平均直径为288 nm。经静电纺丝后，车前素423的活性从5200AU/ml降至25600AU/ml，对腐乳杆菌DSM-20017和屎肠球菌HKLHS的活性分别从204800AU/ml降至5200AU/ml。包裹在纳米纤维中的植物乳杆菌423细胞从电纺前的2.3×10(10)cfu/ml下降到电纺后的4.7×10(8)cfu/ml。包裹在纳米纤维中的细胞继续产生植物素423。这是首次报道将细菌素和植物乳杆菌细胞包裹在纳米纤维中。该方法可用于设计细菌素的给药系统和益生菌乳酸菌的包埋。这项技术目前正在进行优化。
Plantaricin 423, produced by Lactobacillus plantarum 423, was encapsulated in nanofibers that were produced by the electrospinning of 18% (w/v) polyethylene oxide (200 000 Da). The average diameter of the nanofibers was 288 nm. Plantaricin 423 activity decreased from 51 200 AU/ml to 25 600 AU/ml and from 204 800 AU/ml to 51 200 AU/ml after electrospinning, as determined against Lactobacillus sakei DSM 20017 and Enterococcus faecium HKLHS, respectively. Cells of L. plantarum 423 encapsulated in nanofibers decreased from 2.3 x 10(10) cfu/ml before electrospinning to 4.7 x 10(8) cfu/ml thereafter. Cells entrapped in the nanofibers continued to produce plantaricin 423. This is the first report on the encapsulation of a bacteriocin and cells of L. plantarum in nanofibers. The method may be used to design a drug delivery system for bacteriocins and the encapsulation of probiotic lactic acid bacteria. The technology is currently being optimized.