Single-base mutation detection using neutravidin-modified polystyrene nanoparticle aggregation
Single-base mutation detection using neutravidin-modified polystyrene nanoparticle aggregation
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DOI：
10.2116/analsci.20.893
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发表时间：
2004-06-01
影响因子：
1.6
通讯作者：
Maeda, M
中科院分区：
文献类型：
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作者：
Sato, K;Sawayanagi, M;Maeda, M
描述了使用中性抗生物素蛋白修饰的聚苯乙烯纳米颗粒的非交联聚集的单碱基突变测定。该方法只需要两个步骤：生物素标记的探针和样品DNA的杂交，然后与中性亲和素修饰的纳米颗粒混合。总共在20分钟内检测到聚集。DNA非交联聚集和生物素-亲和素技术的组合使得单核苷酸多态性的检测具有足够的性能。
A single-base mutation assay using the non-crosslinking aggregation of neutravidin-modified polystyrene nanoparticles is described. This method requires only two steps: hybridization of biotinylated probe and sample DNAs, and then mixing with neutravidin-modified nanoparticles. The aggregation was detected within 20 min in total. A combination of the DNA non-crosslinking aggregation and biotin-avidin technology has allowed sufficient performance for the detection of single nucleotide polymorphisms.