Induction of maturation in small oocytes from sexually immature mice by fusion with meiotic or mitotic cells.
Induction of maturation in small oocytes from sexually immature mice by fusion with meiotic or mitotic cells.
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通过与减数分裂或有丝分裂细胞融合，诱导性成熟小鼠的小卵母细胞成熟。
DOI：
10.1016/0014-4827(78)90534-7
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发表时间：
1978
影响因子：
3.7
通讯作者：
H. Bałakier
中科院分区：
文献类型：
--
作者：
H. Bałakier
在灭活仙台病毒的帮助下，将不能在体外恢复减数分裂的性未成熟小鼠的核团状卵母细胞与成熟卵母细胞（进入中期I）或有丝分裂停滞的4细胞胚胎的卵裂球融合。在杂种细胞中，未成熟卵母细胞的萌发囊泡在融合后不久从网状期进展到中期I，这表明它能够响应来自减数分裂和有丝分裂细胞的染色体凝聚因子。因此，这些卵母细胞不能启动成熟可能是由于它们的细胞质中缺乏成熟促进因子或成熟促进因子水平不足。
Dictyate oocytes from sexually immature mice which are incompetent to resume meiosis in vitro were fused with the help of inactivated Sendai virus with maturing oocytes (entering metaphase I) or with the blastomeres of 4-cell embryos arrested in mitosis. In hybrid cells the germinal vesicle from immature oocytes progressed shortly after fusion from dictyate to metaphase I which shows that it is capable of responding to a chromosome condensation factor from both meiotic and mitotic cells. Thus the incompetence of these oocytes to initiate maturation may be due to the absence or the insufficient level of a maturation promoting factor in their cytoplasm.