PPAR Nuclear Receptors and Altered RPE Lipid Metabolism in Age-Related Macular Degeneration
PPAR Nuclear Receptors and Altered RPE Lipid Metabolism in Age-Related Macular Degeneration
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DOI：
10.1007/978-1-4419-1399-9_49
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发表时间：
2010-01-01
期刊：
影响因子：
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通讯作者：
Cousins, Scott
中科院分区：
文献类型：
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作者：
Malek, Goldis;Hu, Peng;Cousins, Scott
早期干性老年性黄斑变性(ARMD)以富含脂肪和蛋白质的视网膜下沉积为特征，其病理生理机制仍很不清楚。脂质和细胞外基质(ECM)分子在视网膜下色素上皮(RPE)沉积和Bruch膜中的积聚和代谢失调在ARMD中起着一定的作用。流行病学研究表明，饮食中摄入较多的长链多不饱和脂肪酸(LCPUFA)会增加罹患这种疾病的风险。更具体地说，n-6脂肪酸与n-3脂肪酸相比更是如此。多不饱和脂肪酸是脂质过氧化的膜靶标和核受体的天然配体，即过氧化物酶体增殖物激活受体(PPAR)。在这里，我们研究了在有或没有脂肪酸的情况下，与脂代谢有关的基因的表达以及PPAR的三种异构体在人类RPE永生化细胞系(ARPE19)中的表达。
The pathophysiology of 'early' dry age-related macular degeneration (ARMD), characterized by the accumulation of lipid and protein-rich sub-retinal deposits remains largely unknown. Accumulation and dysregulated turnover of lipids as well as extracellular matrix (ECM) molecules in sub-retinal pigment epithelial (RPE) deposits and Bruch's membrane, itself an ECM, play a role in ARMD. Epidemiological studies have shown an increased risk for the disease associated with higher dietary intake of long chain poly-unsaturated fatty acids (LCPUFA.) and specifically more so for n-6 versus n-3 fatty acids. PUFAs are membrane targets of lipid peroxidation and natural ligands for the nuclear receptors, peroxisome proliferator activated receptors (PPAR). Here we investigated the expression of genes involved in lipid metabolism and expression of the three isoforms of PPARs in an immortalized cell line of human RPE cells (ARPE19) in the presence or absence of fatty acids.